Laxative effects and mechanisms of echinacoside on constipation mice
AIM To investigate the laxative effects of echinacoside on constipation mice and the mechanisms.METHODS KM mice divided into the control group,the model group and the low and high doses echinacoside groups(30,70 mg/kg),with 8 mice in each group,were induced into constipation models by gavage feeding of loperamide except for those of the control group.The mice had their defecation frequency,counts of fecal particles and fecal water content recorded;their rate of small intestine transport evaluated by barium meal propulsion method;their colonic morphology observed by HE staining;their colonic activities of SOD,GSH-Px and the levels of IL-1β and TNF-α detected by kit;their colonic AQP3 protein expression detected by immunofluorescence;and their colonic protein expressions of SR4,SP,VIP,AQP3,CFTR and PKA detected by Western blot.RESULTS Compared with the model group,both echinacoside groups displayed increased defecation frequency,counts of fecal particles,fecal water content,intestinal transport rate,colonic activities of SOD and GSH-Px,protein expressions of SR4,SP,AQP3,CFTR and PKA(P<0.05,P<0.01);improved colonic pathological injury;and decreased levels of IL-1β,TNF-α,and VIP protein expression(P<0.05,P<0.01).CONCLUSION Being a laxative to constipation mice,echinacoside may prompt reduction of intestinal oxidative stress and inflammatory reaction via improvement of intestinal neurotransmitter abnormality and colonic AQP3 expression.
国家科技期刊平台
NSTL
万方数据
维普
