摘要
目的 建立UPLC法同时测定外感风痧颗粒中绿原酸、原儿茶酸、原儿茶醛、隐绿原酸、丁香酸、1,3-二咖啡酰奎宁酸、新绿原酸、木犀草苷、异绿原酸 B、异绿原酸 A、橙皮苷、咖啡酸、异绿原酸 C、迷迭香酸的含量.方法 分析采用Waters ACQUITY UPLC.HSS C18 色谱柱(2.1 mm×100 mm,1.8 μm);流动相乙腈-0.2%磷酸,梯度洗脱;体积流量0.2 mL/min;柱温 30℃;检测波长 275、327 nm.再进行化学计量学分析.结果 14 种成分在各自范围内线性关系良好(r≥0.999 6),平均加样回收率 95.68%～104.8%,RSD 0.7%～3.0%.7 批样品聚为 2 类,3 种主成分累积贡献率达93.031%.结论 该方法准确可靠,可用于外感风痧颗粒的质量控制.
Abstract
AIM To establish a UPLC method for the simultaneous content determination of chlorogenic acid,protocatechuic acid,protocatechualdehyde,cryptochlorogenic acid,syringic acid,1,3-dicaffeoylquinic acid,cynaroside,isochlorogenic acid B,neochlorogenic acid,isochlorogenic acid A,hesperidin,caffeic acid,isochlorogenic acid C and rosmarinic acid in Waigan Fengsha Granules.METHODS The analysis was performed on a 30℃ thermostatic Waters ACQUITY UPLC.HSS C18 column(2.1 mm×100 mm,1.8 μm),with the mobile phase comprising of acetonitrile-0.2%phosphoric acid flowing at 0.2 mL/min in a gradient elution manner,and the detection wavelengths were set at 275,327 nm.Subsequently,chemometric analysis was performed.RESULTS Fourteen constituents showed good linear relationships within their own ranges(r≥0.999 6),whose average recoveries were 95.68%-104.8%with the RSDs of 0.7%-3.0%.Seven batches of samples were clustered into two types,three principal components demonstrated the acumulative contribution rate of 93.031%.CONCLUSION This accurate and reliable method can be used for the quality control of Waigan Fengsha Granules.
基金项目
广西自然科学基金(2020GXNSFBA297135)
广西壮族自治区高等学校中药提取纯化与质量分析重点实验室项目(桂教科研[2014]6号)
维普
万方数据