HPLC-MS/MS法同时测定月季花配方颗粒中10种成分的含量

Simultaneous content determination of ten constituents in Yuejihua Formula Granules by HPLC-MS/MS

李志娇 ¹郑振秋 ¹张晓博 ²巩长芹¹

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作者信息

1. 临沂市检验检测中心,山东临沂 276001
2. 齐鲁制药有限公司,山东济南 250000
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摘要

目的建立HPLC-MS/MS法同时测定月季花配方颗粒中金丝桃苷、异槲皮苷、没食子酸、鞣花酸、槲皮苷、芦丁、阿福豆苷、萹蓄苷、槲皮素-3-O-6-反式-香豆酰基-β-D-葡萄糖苷、槲皮素 3-O-2″-没食子酰基-β-D-葡萄糖苷的含量.方法分析采用Waters Acquity UPLC HSS T3 色谱柱(1.8 μm,2.1 mm×150 mm);流动相甲醇-0.1%甲酸,梯度洗脱;体积流量 0.3 mL/min;柱温 40℃;电喷雾离子源;负离子扫描;多反应监测模式.结果 10 种成分在各自范围线性关系良好(r＞0.995 8),平均加样回收率97.3%～99.6%,RSD 0.8%～2.7%.结论该方法简便快速,灵敏度高,专属性强,可用于月季花配方颗粒的质量控制.

Abstract

AIM To establish an HPLC-MS/MS method for the simultaneous content determination of hyperoside,isoquercitrin,gallic acid,ellagic acid,quercetin,rutin,afzelin,avicularin,quercetin-3-O-6-trans-chumaryl-β-D-glucoside and quercetin 3-O-2″-galloyl-β-D-glucoside in Yuejihua Formula Granules.METHODS The analysis was performed on a 40℃ thermostatic Water Acquisition UPLC ® HSS T3 column(1.8 μm,2.1 mm×150 mm),with the mobile phase comprising of methanol-0.1%formic acid flowing at 0.3 mL/min in a gradient elution manner,and electron spray ionization source was adopted in negative ion scanning with multiple reaction monitoring mode.RESULTS Ten constituents showed good linear relationships within their own ranges(r＞0.995 8),whose average recoveries were 97.3%-99.6%with the RSDs of 0.8%-2.7%.CONCLUSION This simple,rapid,sensitive and specific method can be used for the quality control of Yuejihua Formula Granules.

关键词

月季花配方颗粒/化学成分/含量测定/HPLC-MS/MS

Key words

Yuejihua Formula Granules/chemical constituents/content determination/HPLC-MS/MS

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基金项目

山东省药品质量风险监测工作专项(2022)(鲁食药检业[2022]37号)

出版年

2024

中成药

国家食品药品监督管理局,信息中心中成药信息站,上海中药行业协会

中成药

CSTPCD北大核心

影响因子：1.217

ISSN：1001-1528

参考文献量21

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