摘要
目的 研究棒柄花Cleidion brevipetiolatum pax et Hoffm.化学成分及其抗炎活性.方法 棒柄花提取物采用硅胶、MCI、Rp-18、Sephadex LH-20、制备型高效液相色谱进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构.采用MTT法和Griess法测定各化合物的抗炎活性.结果 从中分离得到 17 个化合物,分别鉴定为臭矢菜素C(1)、莨菪亭(2)、秦皮素(3)、异秦皮啶(4)、木犀草素(5)、芹菜素(6)、金圣草黄素(7)、1-羟基-2-O-β-D吡喃葡萄糖苷-4-烯丙基苯(8)、1-O-β-D-glucopyranosyl-4-allylbenzene(9)、反式-1-(4-丙烯基)-苯酚-β-D-吡喃葡萄糖苷(10)、苄基葡萄糖苷(11)、2-苯乙基-1-O-β-D-吡喃葡萄糖苷(12)、(+)-丁香脂素(13)、橙皮酰胺(14)、(S)-(+)-2-顺式-脱落酸(15)、黑麦草内酯(16)和hydroxychavicol(17).棒柄花醇提物和乙酸乙酯部位抑制NO的IC50值分别为(44.11±5.29)、(24.25±3.59)μg/mL;化合物 2、5～7 抑制NO的IC50 值为 3.55～14.53 μmol/L.结论 化合物 1～7、13～17 为首次从该植物分离得到.该植物中的简单香豆素类和黄酮类化合物具有良好的NO抑制作用.
Abstract
AIM To study the chemical constituents from Cleidion brevipetiolatum pax et Hoffm.and their anti-inflammatory activities.METHODS The extract from C.brevipetiolatum was isolated and purified by silica gel,MCI,Rp-18,Sephadex LH-20,preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The MTT and Griess methods were used to evaluate the anti-inflammatory activities of compounds.RESULTS Seventeen compounds were isolated and identified as cleomiscosin C(1),scopoletin(2),fraxedin(3),isofraxidin(4),luteolin(5),apigenin(6),chrysoeriol(7),1-hydroxy-2-O-β-D-glucopyranosyl-4-allylbenzene(8),1-O-β-D-glucopyranosyl-4-allylbenzene(9),trans-1-(4-propenyl)-phenol-β-D-glucopyranosyl(10),benzyl-O-β-D-glucopyranoside(11),2-phenylethyl-1-O-β-D-glucopyranoside(12),(+)-syringaresinol(13),aurantiamide(14),(S)-(+)-2-cis-abscisic acid(15),loliolide(16),and hydroxychavicol(17).The ethanol extract of C.brevipetiolatum and its ethyl acetate portion showed NO inhibition with IC50 values of(44.11±5.29),(24.25±3.59)μg/mL,respectively.The IC50 values of compounds 2,and 5-7 were 3.55-14.53 μmol/L.CONCLUSION Compounds 1-7 and 13-17 are isolated from this plant for the first time.Simple coumarins and flavones from this plant show good inhibition of the production of NO.
基金项目
国家自然科学基金(82060783)
广西自然科学基金(2020GXNSFAA259001)
桂林医学院硕士研究生科研项目(GYYK2023008)
维普
万方数据