摘要
目的 研究地骨皮甲醇部位的化学成分及其抗炎活性.方法 地骨皮 70%甲醇部位采用各种现代色谱学方法分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构;建立RAW264.7 细胞炎症模型评价化合物的体外抗炎活性.结果 从中分离得到了 11 个化合物,分别鉴定为汉黄芩素(1)、汉黄芩素 7-O-β-D-乙基葡萄糖醛酸苷(2)、汉黄芩素 7-O-β-D-甲基葡萄糖醛酸苷(3)、(-)-丁香树脂酚(4)、表松脂素-4′-O-β-D-葡萄糖苷(5)、丁香酸葡萄糖苷(6)、3-(2-羟苯基)-丙酸甲酯(7)、3-(2-羟苯基)-丙酸乙酯(8)、丁香醛(9)、邻苯二酚(10)、(E)-3-(3,4-dimethoxyphenyl)-N-phenethylacrylamide(11).相较于模型组,化合物 1～3 和 11 给药后,NO生成量降低(P＜0.05,P＜0.01).结论 化合物 2～5、7～8 和 11 为首次从枸杞属植物中分离得到.化合物 1～3 和 11 具有显著抗炎活性,化合物 2 的抗炎活性最好.
Abstract
AIM To study chemical constituents of methanol fraction from Lycii Cortex and their anti-inflammatory activities.METHODS The 70%ethanol extract of Lycii Cortex was isolated and purified by various modern chromatographic techniques,the structures of obtained compounds were identified by physicochemical properties and spectral data.Their anti-inflammatory activities in vitro were evaluated by RAW264.7 cell inflammation model.RESULTS Eleven compounds were isolated and identified as wogonin(1),wogonin7-O-β-D-ethylglucuronic acid(2),wogonin7-O-β-D-methylglucuronic acid(3),(-)-syringaresinol(4),(-)-Pinoresinol 4-O-β-D-glucopyranoside(5),glucosyringic acid(6),methyl 3-(2-hydroxyphenyl)propionate(7),ethyl melilotate(8),syringaldehyde(9),catechol(10),and(E)-3-(3,4-dimethoxyphenyl)-N-phenethylacrylamide(11).Compared with the model group,NO production was reduced after administration of compounds 1-3 and 11(P＜0.05,P＜0.01).CONCLUSION Compounds 2-5,7-8,and 11 are isolated from Lycium genus for the first time.Compounds 1-3,and 11 have significant anti-inflammatory activities,and compound 2 has the best anti-inflammatory activity.
基金项目
南京市中医药科技专项(2022)(ZYYB202201)
南京市中医药科技专项(2022)(ZYYB202203)
维普
万方数据