Simultaneous content determination of eleven constituents and their inhibitory effects on pancreatic lipase for Sanzi Yangqin Decoction
AIM To simultaneously determine the contents of 3,6'-disinapoyl sucrose,sibiricose A5,sibiricose A6,ferulic acid,sinapic acid,caffeic acid,luteolin,progoitrin,apigenin,isorhamnetin and 3,4-dihydroxybenzaldehyde in Sanzi Yangqin Decoction,and to investigate their inhibitory effects on pancreatic lipase.METHODS The high-performance liquid chromatography-triple quadrupole tandem mass spectrometry(HPLC-QQQ-MS/MS)analysis was performed on a 25 ℃ thermostatic Halo-C18column(2.1 mmx100 mm,2.7 μm),with the mobile phase comprising of water(containing 0.05％formic acid)-acetonitrile(containing 0.05％formic acid)flowing at 0.3 mL/min in a gradient elution manner,and electron spray ionization source was adopted in negative ion scanning with multiple reaction monitoring mode.The simulation was performed by molecular docking,after which 4-nitrophenyl butyrate method was adopted in the evaluation of inhibitory effects.RESULTS Eleven constituents showed good linear relationships within their own ranges(R2＞0.992 0),whose average recoveries were 85.79％-117.52％with the RSDs of 0.78％-3.73％.3,6'-Disinapoyl sucrose,caffeic acid,sinapic acid and 3,4-dihydroxybenzaldehyde demonstrated good affinities with pancreatic lipase,the IC50 values of Sanzi Yangqin Decoction,caffeic acid,sinapic acid and 3,4-dihydroxybenzaldehyde were 41.16 mg/mL,4.006 mmol/L,6.798 mmol/L and 10.48 mmol/L,respectively.CONCLUSION This stable and reliabe method can provide theoretical basis for the quality control of Sanzi Yangqin Decoction with inhibitory effects on pancreatic lipase.
Sanzi Yangqin Decoctionchemical constituentscontent determinationpancreatic lipasehigh-performance liquid chromatography-triple quadrupole tandem mass spectrometry(HPLC-QQQ-MS/MS)molecular docking4-nitrophenyl butyrate method
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维普
