首页|玉屏风散对肺癌细胞PD-L1表达及T细胞功能的影响

玉屏风散对肺癌细胞PD-L1表达及T细胞功能的影响

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目的 探讨玉屏风散对程序性死亡受体1(PD-1)-程序性死亡配体-1(PD-L1)结合及对肺癌细胞在体内外生长的影响.方法 MTT法检测玉屏风散提取物对小鼠Lewis肺癌LLC细胞生长的影响;乳酸脱氢酶(LDH)释放实验检测玉屏风散提取物对T细胞和LCC细胞共培养中靶细胞杀伤性作用;酶联免疫吸附(ELISA)实验检测玉屏风散提取物对PD-1和PD-L1结合的阻断作用,以及对γ干扰素(IFN-γ)和肿瘤坏死因子-α(TNF-α)释放水平的影响;同基因肿瘤模型分析玉屏风散对肺癌LLC细胞体内生长的抑制作用;免疫组化染色考察玉屏风散对肿瘤组织内CD4+T细胞、CD8+T细胞浸润的影响.结果 玉屏风散呈剂量依赖性抑制LLC细胞的生长及内源性PD-L1蛋白表达(P<0.05,P<0.01),竞争性阻断 PD-1 和 PD-L1 结合(P<0.05,P<0.01),促进 T 细胞 IFN-γ和 TNF-α 的释放(P<0.05,P<0.01),阻断PD-L1对T细胞功能性抑制作用(P<0.05,P<0.01).玉屏风散可以抑制LLC肿瘤生长(P<0.01),增加CD4+T、CD8+T细胞的浸润数量(P<0.01).结论 玉屏风散在体外细胞模型中可阻断PD-L1对T细胞功能的抑制作用,在同源小鼠肿瘤模型中具有抗肿瘤效果,可增强CD4+T细胞、CD8+T细胞对癌细胞的杀伤作用.
Effects of Yupingfeng Powder on PD-L1 expression and T cell function in lung cancer cells
AIM To explore the effects of Yupingfeng Powder on the binding of programmed death receptor 1(PD-1)and programmed death ligand 1(PD-L1),and the growth of lung cancer cells in vitro and in vivo.METHODS Yupingfeng Powder extract had its effect on the growth of Lewis lung carcinoma(LLC)cells in mice detected by MTT assay;its effect on eliminating target cells in the co-culture of T cells and LCC cells detected by lactate dehydrogenase(LDH)release test;its effect on blocking the combination of PD-1 and PD-L1,and its influence on the levels of interferon-γ(IFN-γ)and tumor necrosis factor-α(TNF-α)release detected by enzyme-linked immunosorbent assay(ELISA);its inhibitory effect on the growth of LLC cells in vivo analyzed by homologous tumor model;its effect on the infiltration of CD4+T cells and CD8+T cells in tumor tissues investigated by immunohistochemical staining.RESULTS Yupingfeng Powder inhibited the growth of LLC cells and the expression of endogenous PD-L1 protein in a dose-dependent manner(P<0.05,P<0.01),competitively blocked the combination of PD-1 and PD-L1(P<0.05,P<0.01),and promoted the release of IFN-γ and TNF-α from T cells(P<0.05,P<0.01).Yupingfeng Powder inhibited the growth of LLC tumor(P<0.01),and increased the infiltration of CD4+T and CD8+T cells(P<0.01).CONCLUSION Yupingfeng Powder exhibits its in vitro capability in blocking the PD-L1 inhibition on T cells,and its in vivo anti-tumor effect in terms of the improvement of CD4+T cells and CD8+T cells in this homologous mouse tumor model.

Yupingfeng Powderlung cancerLLC cellsT cellsPD-1PD-L1

叶震中、李世杰、牛彩琴

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川北医学院附属医院中医科,四川南充 637000

成都中医药大学附属医院肿瘤科,四川成都 610000

玉屏风散 肺癌 LLC细胞 T细胞 PD-1 PD-L1

国家自然科学基金项目四川省中医药管理局面上项目

815739682023M5340

2024

中成药
国家食品药品监督管理局,信息中心中成药信息站,上海中药行业协会

中成药

CSTPCD北大核心
影响因子:1.217
ISSN:1001-1528
年,卷(期):2024.46(6)