北柴胡质量控制研究
Quality contol of Bupleurum chinense
于静波 1韩越 2艾启迪 2孙洋 2周梓洋 1刘赛虎 2张子浩 2舒慧 2王宇红1
作者信息
- 1. 湖南中医药大学科技创新中心/中药粉体与创新药物省部共建国家重点实验室培育基地,湖南 长沙 410208
- 2. 湖南中医药大学药学院,湖南 长沙 410208
- 折叠
摘要
目的 对北柴胡质量进行控制.方法 分析采用Venusil XBP C18色谱柱(250 mm×4.6 mm,5 μm);流动相乙腈-水,梯度洗脱;体积流量 1.0 mL/min;柱温 35℃;检测波长210 nm.建立HPLC指纹图谱,测定柴胡皂苷A、柴胡皂苷B2、柴胡皂苷C、柴胡皂苷D、柴胡皂苷E、柴胡皂苷F、6″-O-乙酰基柴胡皂苷A 的含量,进行主成分分析.结果 12 批药材指纹图谱中有 13 个共有峰,相似度 0.970～0.995.7 种成分在各自范围内线性关系良好(R2≥0.999 8),平均加样回收率 90.75%～100.91%,RSD 1.6%～4.0%.内蒙古、山西产药材中各成分含量接近.结论 该方法精密、准确、稳定,可用于北柴胡质量评价.
Abstract
AIM To control the quality of Bupleurum chinense DC.METHODS The analysis was performed on a 35℃ thermostatic Venusil XBP C18 column(250 mm×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-water flowing at 1.0 mL/min,and the detection wavelength was set at 210 nm.The HPLC fingerprints were established,after which the contents of saikosaponin A,saikosaponin B2,saikosaponin C,saikosaponin D,saikosaponin E,saikosaponin F and 6″-O-acetylsaikosaponin A were determined,and principal component analysis was made.RESULTS There were thirteen common peaks in the fingerprints for twelve batches of medicinal materials with the similarities of 0.970-0.995.Seven constituents showed good linear relationships within their own ranges(R2≥0.999 8),whose average recoveries were 90.75%-100.91% with the RSDs of 1.6%-4.0% .Various constituents demonstrated similar contents in medicinal materials originated in Inner Mongolia and Shanxi.CONCLUSION This precise,accurate and stable method can be used for the quality evaluation of B.chinense.
关键词
北柴胡/质量控制/HPLC指纹图谱/含量测定/主成分分析Key words
Bupleurum chinense DC./quality control/HPLC fingerprints/content determination/principal component analysis引用本文复制引用
基金项目
国家自然科学基金项目(82204613)
中药粉体与创新药物省部共建国家重点实验室培育基地开放基金项目(科函[2020]8号)
湖南省自然科学基金项目(2020JJ5415)
湖南省自然科学基金项目(2021JJ40410)
湖南中医药大学一流学科开放基金项目(2020ZYX06)
湖南中医药大学一流学科开放基金项目(2021ZYX25)
出版年
2024
国家科技期刊平台
NSTL
万方数据