摘要
目的 建立UPLC-QTOF/MS法分析乳宁颗粒化学成分及入血成分.方法 2 只大鼠灌胃给予颗粒水溶液,于0、0.5、1、2、4 h采血.分析采用Acquity UPLC BEH C18色谱柱(100 mm×2.1 mm,1.7 μm);流动相 0.1%甲酸-乙腈(正离子)或水-乙腈(负离子),梯度洗脱;体积流量 0.3 mL/min;柱温 40℃;电喷雾离子源;正负离子扫描.结果 共鉴定出 166 种化学成分,包括 44 种黄酮类、17 种萜类、23 种生物碱类、11 种皂苷类、29 种酚酸类、13 种醌类、10 种有机酸类、19 种其他类,以及 11 种入血成分.结论 该方法准确稳定,可为乳宁颗粒临床应用提供数据支撑,也能为其后续质量控制、物质基础研究奠定基础.
Abstract
AIM To establish a UPLC-QTOF/MS method for the analyses of chemical constituents and constituents absorbed into blood from Runing Granules.METHODS Two rats were given intragastric administration of the aqueous solution of granules,after which blood collection was made at 0,0.5,1,2,4 h.The analysis was performed on a 40℃ thermostatic Acquity UPLC BEH C18 column(100 mm×2.1 mm,1.7 μm),with the mobile phases comprising of 0.1% formic aci D-acetonitrile(positive ion)or water-acetonitrile(negative ion)flowing at 0.3 mL/min in a gradient elution manner,and electron spray ionization source was adopted in positive and negative ion scanning.RESULTS Total 166 chemical constituents were identified,containing 44 flavonoids,17 terpenes,23 alkaloids,11 saponins,29 phenolic acids,13 quinones,10 organic acids,and 19 others,along with 11 constituents absorbed into blood.CONCLUSION This accurate and stable method can provide a data support for the clinical application of Runing Granules,and lay foundations for the further researches on their quality control and material basis.
基金项目
江西省中医药管理局科技计划项目(2023B1357)
国家科技期刊平台
NSTL
万方数据