基于PTEN/PI3K/Akt信号通路探讨糖通饮对糖尿病肾病大鼠足细胞损伤的保护作用
Protective effects of Tangtongyin on podocyte injury in rats with diabetic nephropathy via PTEN/PI3K/Akt signaling pathway
杨红 1潘艳伶 2陈洪民 1伏红颖 3唐露 1凌湘力2
作者信息
- 1. 贵州医科大学,贵州 贵阳 550004
- 2. 贵州医科大学附属医院,贵州 贵阳 550004
- 3. 四川大学华西医院龙泉医院,四川 成都 610000
- 折叠
摘要
目的 探讨糖通饮对糖尿病肾病(DN)大鼠足细胞损伤的保护作用.方法 高脂高糖饮食联合链脲佐菌素(STZ)多次注射建立DN大鼠模型,并随机分为模型组、厄贝沙坦组(13.5 mg/kg)和糖通饮低、中、高剂量组(930、1 860、3 720 mg/kg),另设置正常组,各组灌胃给予相应剂量药物,每天 1 次,连续 8 周.检测各组大鼠空腹血糖(FBG)、24 h尿蛋白(24-h UP)、血肌酐(Scr)、血尿素氮(BUN)水平,HE染色观察各组大鼠肾组织病理形态学改变,透射电子显微镜观察肾足细胞超微结构变化,RT-qPCR 法检测肾组织 PTEN、PI3K、Akt、nephrin、podocin、CD2AP mRNA表达,Western blot法检测肾组织PTEN、PI3K、Akt、p-Akt、nephrin、podocin、CD2AP蛋白表达.结果 与模型组比较,糖通饮各剂量组FBG、24-h UP、Scr、BUN水平降低(P＜0.05,P＜0.01),肾组织病理形态和足细胞超微结构得到改善,PTEN、nephrin、podocin、CD2AP mRNA和蛋白表达升高(P＜0.05,P＜0.01),p-Akt蛋白表达降低(P＜0.05,P＜0.01);糖通饮中、高剂量组PI3K、Akt mRNA和蛋白表达降低(P＜0.05,P＜0.01).结论 糖通饮对DN大鼠肾组织足细胞有保护作用,其机制可能与调节PTEN/PI3K/Akt信号通路有关.
Abstract
AIM To investigate the protective effects of Tangtongyin on podocyte injury in rats with diabetic nephropathy(DN).METHODS The DN rat models established by feeding of high-fat and high-sugar diet combined with multiple injections of streptozotocin(STZ)were randomly divided into the model group,the irbesartan group(13.5 mg/kg)and the low-dose,medium-dose and high-dose Tangtongyin groups(930,1 860,3 720 mg/kg),in contrast to those normal rats of the blank group.In the 8 weeks consecutive administration,each group was intragastrically dosed with the corresponding drug once daily.The rats had their fasting blood glucose(FBG),24-hour urine protein(24-h UP),serum creatinine(Scr)and blood urea nitrogen(BUN)levels detected;their renal pathological changes observed by HE staining;their ultrastructural changes of renal podocytes observed by transmission electron microscopy;their mRNA expressions of renal PTEN,PI3K,Akt,nephrin,podocin and CD2AP detected by RT-qPCR;and their protein expressions of renal PTEN,PI3K,Akt,p-Akt,nephrin,podocin and CD2AP detected by Western blot.RESULTS Compared with the model group,the Tangtongyin groups displayed decreased levels of FBG,24-h UP,Scr and BUN(P＜0.05,P＜0.01);improved renal pathological morphology and podocyte ultrastructure;increased mRNA and protein expressions of PTEN,nephrin,podocin and CD2AP(P＜0.05,P＜0.01);and decreased expression of p-Akt protein(P＜0.05,P＜0.01).Additionally,the medium-dose and high-dose Tangtongyin groups shared indecreased mRNA and protein expressions of PI3K and Akt(P＜0.05,P＜0.01).CONCLUSION Tangtongyin can protect the renal podocytes in DN rats,and its mechanism may associate with its efficacy via PTEN/PI3K/Akt signaling pathway.
关键词
糖通饮/糖尿病肾病/足细胞/PTEN/PI3K/Akt信号通路Key words
Tangtongyin/diabetic nephropathy/podocyte/PTEN/PI3K/Akt signaling pathway引用本文复制引用
基金项目
国家自然科学基金项目(82060837)
贵州省科技基金委员会合作计划项目(黔科合基础-ZK[2023]一般369号)
2024年度贵州省中医药管理局中医药、民族医药科学技术研究专项课题(QZYY-2024-025)
凌湘力全国名中医传承工作室建设项目(国中医药办人教函[2022]245号)
出版年
2024
国家科技期刊平台
NSTL
万方数据