HPLC法同时测定安神胶囊中7种成分的含量
Simultaneous content determination of seven constituents in Anshen Capsules by HPLC
崔帅 1崔俊凤 1梁秀坤 1王志勇 1夏青2
作者信息
- 1. 滨州市检验检测中心,山东 滨州 256600;滨州市化学药物研发与质量控制重点实验室(筹),山东 滨州 256600
- 2. 齐鲁工业大学(山东省科学院)生物研究所,山东 济南 250103
- 折叠
摘要
目的 建立HPLC法同时测定安神胶囊中斯皮诺素、阿魏酸、迷迭香酸、丹酚酸B、五味子甲素、五味子乙素、五味子醇甲的含量.方法 分析采用Inertsustain C18色谱柱(5 μm,4.6 mm×250 mm);流动相甲醇-乙腈-0.2%磷酸,梯度洗脱;体积流量 1.0 mL/min;柱温 30℃;检测波长 203、270 nm.再进行主成分分析、偏最小二乘判别分析.结果 7 种成分在各自范围内线性关系良好(r＞0.999 0),平均加样回收率 96.12%～102.70%,RSD 0.31%～1.83%.10 批样品按厂家分为2 类,峰5(五味子醇甲)、峰4(丹酚酸B)为质量差异标志物.结论 该方法简便灵敏,专属性强,重复性好,可用于安神胶囊的质量控制.
Abstract
AIM To establish an HPLC method for the simultaneous content determination of spinosin,ferulic acid,rosmarinic acid,salvianolic acid B,schisandrin A,schizandrin B and schisandrol A in Anshen Capsules.METHODS The analysis was performed on a 30℃thermostatic Inertsustain C18 column(5 μm,4.6 mm×250 mm),with the mobile phase comprising of methanol-acetonitrile-0.1%phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 203,270 nm.Subsequently,principal component analysis and partial least squares discriminant analysis were made.RESULTS Seven constituents showed good linear relationships within their own ranges(r＞0.999 0),whose average recoveries were 96.12%-102.70%with the RSDs of 0.31%-1.83%.Ten batches of samples were divided into two categories according to manufacturers,and peaks 5(schisandrol A),4(salvianolic acid B)were quality difference markers.CONCLUSION This simple,sensitive,specific and reproducible method can be used for the quality control of Anshen Capsules.
关键词
安神胶囊/化学成分/含量测定/HPLC/主成分分析/偏最小二乘判别分析Key words
Anshen Capsules/chemical constituents/content determination/HPLC/principal component analysis/partial least squares discriminant analysis引用本文复制引用
基金项目
国家自然科学基金联合基金重点支持项目(U23A20514)
出版年
2024
国家科技期刊平台
NSTL
万方数据