摘要
目的 研究山橿根大极性部位的化学成分及其抗肿瘤活性.方法 山橿根大极性部位采用硅胶柱、Sephadex LH-20 凝胶柱、半制备HPLC、ODS中压柱进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构.采用MTT法测定其抗肿瘤活性.结果 从中分离得到 13 个化合物,分别鉴定为 2,6-二甲氧基-4-羟基苯基-1-O-β-D-葡萄糖苷(1)、3-羟基-4,5-二甲氧基苯酚-β-D-吡喃葡萄糖苷(2)、紫丁香苷(3)、1-O-3,4-二甲氧基-5-羟苯基-(6-O-3,5-二甲氧基没食子酰)-β-D-吡喃葡萄糖苷(4)、p-cymen-7-yl β-D-glucopyranoside(5)、pisumionoside(6)、staphylionoside D(7)、dendranthemoside B(8)、lynoiside(9)、nudiposide(10)、淫羊藿次苷 B1(11)、(2S)-pinocembrin-7-O-(6-O-α-L-rhamnopyranosyl-β-D-glucopyranoside)(12)、(+)-N-(methoxycarbonyl)-N-norboldine(13).化合物 3、13 对人肺癌细胞(A549)、人胃癌细胞(MGC80-3)表现出明显的细胞毒活性.结论 化合物 1～13 为首次从山橿中分离得到.化合物 3、13 具有良好的抗肿瘤活性.
Abstract
AIM To study the chemical constituents from the large polar fraction of the roots of Lindera reflexa Hemsl.and their antitumor activities.METHODS The large polar fraction from the roots of L.reflexa was isolated and purified by silica gel column,Sephadex LH-20 gel column,semi-preparative HPLC and ODS medium pressure column,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The antitumor activities were determined by MTT method.RESULTS Thirteen compounds were isolated and identified as 2,6-dimethoxy-4-hydroxyphenyl-1-O-β-D-glucopyranoside(1),3-hydroxy-4,5-dimethoxyphenol-β-D-glucopyranoside(2),syringin(3),1-O-3,4-dimethoxy-5-hydroxyphenyl-(6-O-3,5-dimethoxygalloyl)-β-D-glucopyranoside(4),p-cymen-7-yl β-D-glucopyranoside(5),pisumionoside(6),staphylionoside D(7),dendranthemoside B(8),lynoiside(9),nudiposide(10),icariside B1(11),(2S)-pinocembrin-7-O-(6-O-α-L-rhamnopyranosyl-β-D-glucopyranoside)(12),(+)-N-(methoxycarbonyl)-N-norboldine(13).Compounds 3 and 13 showed obvious cytotoxicity against human lung cancer cells(A549)and human gastric cancer cells(MGC80-3).CONCLUSION Compounds 1-13 are isolated from the roots of L.reflexa for the first time.Compounds 3 and 13 have good anti-tumor activities.
基金项目
国家自然科学基金项目(81773859)
河南省科技攻关项目(212102310353)
河南省中药材产业技术体系(豫财科[2023]7号)
国家科技期刊平台
NSTL
万方数据