摘要
目的 研究来江藤苯丙素类成分及其抗氧化活性.方法 来江藤乙醇提取物采用Rp-C18、MCI、半制备HPLC、硅胶及Sephadex LH-20 进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构.采用MTT法测定其细胞毒性,DPPH、ABTS+自由基清除法测定其抗氧化活性.结果 从中分离得到 15 个苯丙素类化合物,分别鉴定为(+)松脂素(1)、(-)杜仲树脂酚(2)、(-)丁香脂素(3)、buddlenol D(4)、(7R,7'R,7″S,8S,8'S,8″S)-4',5″-dihydroxy-3,5,3',4″-tetramethoxy-7,9':7',9-diepoxy-4,8″-oxy-8,8'-sesquineo-lignan-7″,9″-diol(5)、狗牙花脂素(6)、hedyotol A(7)、dracunculifoside R(8)、毛蕊花糖苷(9)、异毛蕊花糖苷(10)、连翘酯苷F(11)、异地黄苷(12)、curcasinlignan B(13)、erythro-2,3-bis(4-hydroxy-3-methoxyphenyl)-3-ethoxypropan-l-ol(14)、枸橼苦素 C(15).化合物 1～4、9～10 对HepG2 肝癌细胞无明显细胞毒性;化合物 1、3、9、10、12 对DPPH自由基具有较强的清除活性;化合物 1～3、9～10、12、14 对ABTS+自由基具有较强的清除活性.结论 化合物 1～8、12～15 为首次从来江藤属植物中分离得到.来江藤苯丙素类化合物有较强的抗氧化活性.
Abstract
AIM To study the phenylpropanoids from Brandisia hancei Hook.f.and their antioxidant activities.METHODS The extract from B.hancei was isolated and purified by Rp-C18,MCI,semi-preparative HPLC,silica gel and Sephadex LH-20,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The cytotoxicities was determined by MTT method,and the antioxidant activities were determined by DPPH and ABTS+free radical scavenging methods.RESULTS Fifteen phenylpropanoids were isolated and identified as(+)-pinonesinol(1),(-)-medioresinol(2),(-)-syringaresinol(3),buddlenol D(4),(7R,7'R,7″S,8S,8'S,8″S)-4',5″-dihydroxy-3,5,3',4″-tetramethoxy-7,9':7',9-diepoxy-4,8″-oxy-8,8'-sesquineo-lignan-7″,9″-diol(5),(-)-(7R,7'R,7″R,8S,8'S,8″S)-4',4″-dihydroxy-3,3',3″,5-tetramethoxy-7,9':7',9-diepoxy-4,8″-oxy-8,8'-sesquineolignan-7″,9″-diol(6),hedyotol A(7),dracunculifoside R(8),acteoside(9),isoacteoside(10),arenarioside(11),isomartynoside(12),curcasinlignan B(13),erythro-2,3-bis(4-hydroxy-3-methoxyphenyl)-3-ethoxypropan-l-ol(14),citrusin C(15).Compounds 1-4 and 9-10 had no obvious cytotoxicity to HepG2 hepatoma cells.Compounds 1,3,9,10 and 12 had strong scavenging activities against DPPH radicals.Compounds 1-3,9-10,12 and 14 showed strong scavenging activities against ABTS+radical.CONCLUSION Compounds 1-8 and 12-15 are isolated from genus Brandisia for the first time.The phenylpropanoids from B.hancei show strong antioxidant activities.
基金项目
云南省科学技术厅-云南中医学院应用基础研究联合专项基金(202101AZ070001-218)
云南省南药可持续利用研究重点实验室2021年开放课题(202105AG070012ZD2203)
云南省教育厅治疗痹症中医中药工程研究中心(培育)()
保山市科学技术局科技计划项目(202003)
兴滇英才支持计划项目(20220273)
国家科技期刊平台
NSTL
万方数据