首页|黄芩内生真菌及其次级代谢产物酶抑制活性研究

黄芩内生真菌及其次级代谢产物酶抑制活性研究

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  • 国家科技期刊平台
  • NETL
  • NSTL
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目的 研究黄芩内生真菌及其次级代谢产物的酶抑制活性.方法 选用 6 种不同的培养基,采用组织匀浆法对黄芩内生真菌进行分离纯化,以不同酶为靶点评价次级代谢产物的活性,利用分子生物学结合形态学对高活性菌株进行分类学鉴定,利用现代色谱分离技术对高活性化学成分进行跟踪分离.结果 从黄芩中分离得到 64 株内生真菌,发酵得到 128 个次级代谢产物,对腺苷脱氨酶(ADA)、β-内酰胺酶、酪氨酸酶(TYR)有一定抑制活性的样品分别为占 14.06%、3.91%、18.75%,其中菌株HTS-23-2 显示强TYR抑制活性,分子鉴定结果显示其与Aspergillus flavus有 99%的同源性,并从其发酵样本中跟踪分离到 1 个化合物,被鉴定为曲酸.结论 黄芩内生真菌种类丰富,是发现活性成分的良好资源.
Endophytic fungi from Scutellaria baicalensis and the enzyme inhibitory activities of their secondary metabolites
AIM To study endophytic fungi from Scutellaria baicalensis Georgi.and the enzyme inhibitory activities of their secondary metabolites.METHODS Six different media were used to isolate and purify endophytic fungi from S.baicalensis by tissue homogenate method.The activities of secondary metabolites were evaluated by targeting different enzymes.The highly active strains were identified by molecular biology combined with morphology,and the highly active chemical components were tracked and separated by modern chromatographic separation technology.RESULTS Sixty-four endophytic fungal strains were isolated from S.baicalensis,and one hundred and twenty-eight secondary metabolites were obtained by fermentation.The samples with certain inhibitory activities against adenosine deaminase(ADA),β-lactamase and tyrosinase(TYR)accounted for 14.06%,3.91%and 18.75%,respectively.Strain HTS-23-2 showed high TYR inhibitory activity,and 99%homology with Aspergillus flavus by molecular identification.One compound was isolated from the fermentation samples and identified as kojic acid.CONCLUSION S.baicalensis harbors a rich diversity of endophytic fungi,which serve as a valuable resource for active substances.

Scutellaria baicalensis Georgiendophytic fungimetabolitesenzyme inhibitory activitiestracking separation

李德民、马小弟、王康旭、李媚媛、罗满平、孟瑛瑛、杨爱梅、王蓓、张新国

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兰州理工大学生命科学与工程学院,甘肃省中藏药筛选评价及深加工重点实验室,甘肃 兰州 730050

黄芩 内生真菌 代谢产物 酶抑制活性 跟踪分离

国家自然科学基金甘肃省重点研发计划项目甘肃省重点研发计划项目甘肃省科技专员专项温州市科技局甘肃省青年基金计划

3216002121YF5FA08422YF7NA17622CX8GA1142022Y088322JR5RA299

2024

10.3969/j.issn.1001-1528.2024.08.025

中成药
国家食品药品监督管理局,信息中心中成药信息站,上海中药行业协会

中成药

CSTPCD北大核心
影响因子：1.217
ISSN：1001-1528
年,卷(期)：2024.46(8)
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