摘要
目的 研究黑果小檗内生真菌假丝酵母菌Candida sp.的次生代谢产物.方法 假丝酵母菌发酵提取物次级代谢产物的乙酸乙酯部位、石油醚部位采用硅胶、Sephadex LH-20、制备液相色谱进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构.结果 从中分离得到 18 个化合物,分别鉴定为 1-苯基-1,2-乙二醇(1)、4-羟基苯乙醇(2)、4-羟基苯甲酸(3)、对羟基苯乙酸(4)、间羟基苯乙酸(5)、3-甲基亚砜基丙酸(6)、苯乙酸(7)、(S)-N-nitroso-1-amino-p-hydroxy phenylethanol(8)、2-苯乙酰胺(9)、对羟基苯甲醛(10)、2-(4-羟苯基)-乙酸乙酯(11)、邻苯二甲酸二丁酯(12)、5,5'-dimethoxybiphenyl-2,2'-diol(13)、3-吲哚甲醛(14)、N-乙酰-L-苯丙氨酸(15)、9-hydroxy-10E,12Z-octadecadienoic acid(16)、9-hydroxy-10E,12E-octadecadienoic acid(17)、(6E)-5-methylene-6-tetradecenoic acid(18).结论 化合物 1、3～8、10～18 为首次从假丝酵母菌真菌中分离得到.
Abstract
AIM To study the secondary metabolites from the endophytic fungi Candida sp.of Berberis atrocarpa Schneid.METHODS The ethyl acetate fraction and petroleum ether fraction from the secondary metabolites of Candida sp.fermentation extract were separated and purified by silica gel,Sephadex LH-20 and preparative liquid chromatography,then the structures of obtained compounds were identified by physicochemical properties and spectral data.RESULTS Eighteen compounds were isolated and identified as 1-phenyl-1,2-ethanediol(1),4-hydroxyphenethyl alcohol(2),4-hydroxybenzoic acid(3),4-hydroxyphenylacetic acid(4),3-hydroxyphenylacetic acid(5),3-methylsulfinyl propionic acid(6),phenylacetic acid(7),(S)-N-nitroso-1-amino-p-hydroxy phenylethanol(8),2-phenylacetamide(9),p-hydroxybenzaldehyde(10),ethyl 2-(4-hydroxyphenyl)acetate(11),dibutyl phthalate(12),5,5'-dimethoxybiphenyl-2,2'-diol(13),3-indolealdehyde(14),N-acetyl-L-phenylalanine(15),9-hydroxy-10E,12Z-octadecadienoic acid(16),9-hydroxy-10E,12E-octadecadienoic acid(17),(6E)-5-methylene-6-tetradecenoic acid(18).CONCLUSION Compounds 1,3-8 and 10-18 are isolated from Candida sp for the first time.
基金项目
新疆维吾尔自治区高校科研计划(XJEDU2018I018)
维普
万方数据