摘要
目的 建立UPLC-MS/MS法同时测定桑白皮中绿原酸、桑皮苷A、二氢桑色素、氧化白藜芦醇、槲皮素、山柰酚、桑呋喃G、桑黄酮G、桑根酮C、桑根酮D、桑辛素的含量.方法 分析采用Brownlee SPP C18色谱柱(2.1 mm×100 mm,2.7μm);流动相乙腈(含0.1％甲酸)-5 mmol/L乙酸铵,梯度洗脱;体积流量0.5 mL/min;柱温35℃;电喷雾离子源;负离子扫描;多反应监测模式.结果 11种成分在各自范围内线性关系良好(R2>0.9950),平均加样回收率94.53％～103.58％,RSD 2.51％～6.92％.18批药材中均未检测到山柰酚,氧化白藜芦醇、槲皮素、桑呋喃G、桑黄酮G、桑辛素主要存在于外粗皮中,不同产地药材中桑皮苷A含量存在明显差异.结论 该方法稳定、快速、灵敏,可用于桑白皮质量控制.
Abstract
AIM To establish a UPLC-MS/MS method for the simultaneous content determination of chlorogenic acid,mulberroside A,dihydromorin,oxyresveratrol,quercetin,kaempferol,mulberrofuran G,kuwanon G,sanggenon C,sanggenone D and morusin in Mori Cortex.METHODS The analysis was performed on a 35 ℃ thermostatic Brownlee SPP C18 column ( 2.1 mm × 100 mm,2.7 μm ),with the mobile phase comprising of acetonitrile ( containing 0.1％ formic acid)-5 mmol/L ammonium acetate flowing at 0.5 mL/min in a gradient elution manner,and electron spray ionization source was adopted in negative ion scanning with multiple reaction monitoring mode.RESULTS Eleven constituents showed good linear relationships within their own ranges (R2>0.9950),whose average recoveries were 94.53％-103.58％ with the RSDs of 2.51％-6.92％.Kaempferol was not detected in 18 batches of medicinal materials,oxyresveratrol,quercetin,mulberrofuran G,kuwanon G and morusin mainly existed in the outer crude bark,and mulberroside A content demonstrated obvious difference in different producing areas of medicinal materials.CONCLUSION This stable,rapid and sensitive method can be used for the quality control of Mori Cortex.
维普
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