清脂化瘀方HPLC-CAD特征图谱建立及6种糖类含量测定

Establishment of HPLC-CAD characteristic chromatograms and content determination of six carbohydrates for Qingzhi Huayu Formula

张海波 ¹付庆帅 ²姚耿圳 ³周华 ⁴李荣胜 ²杨涛¹

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作者信息

1. 上海中医药大学附属曙光医院心血管研究室,上海 201203;上海市中医临床重点实验室,上海201203
2. 上海黄海制药有限责任公司,上海200942
3. 广州中医药大学第二附属医院,广东省中医院心内科,广州510000
4. 上海中医药大学附属曙光医院心血管研究室,上海 201203
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摘要

目的建立清脂化瘀方HPLC-CAD特征图谱,并测定鼠李糖、果糖、甘露醇、D-无水葡萄糖、蔗糖、棉子糖的含量.方法分析采用Dikma Polyamino HILIC色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-水,梯度洗脱;体积流量0.8 mL/min;柱温35℃.结果 15批样品特征图谱中有14个特征峰相似度大于0.90.6种糖类在各自范围内线性关系良好(r>0.9950),平均加样回收率93.34％～105.64％,RSD 0.5％～2.6％.结论该方法准确可靠,灵敏度高,重复性好,可用于清脂化瘀方糖类的质量控制.

Abstract

AIM To establish the HPLC-CAD characteristic chromatograms of Qingzhi Huayu Formula,and to determine the contents of rhamnose,fructose,mannitol,D-anhydrous glucose,sucrose and raffinose.METHODS The analysis was performed on a 35 ℃ thermostatic Dikma Polyamino HILIC column (4.6 mm×250 mm,5 μm),with the mobile phase comprising of acetonitrile-water flowing at 0.8 mL/min in a gradient elution manner.RESULTS There were 14 common peaks in the characteristic chromatograms of 15 batches of samples with the similarities of more than 0.90.Six carbohydrates showed good linear relationships within their own ranges ( r>0.9950 ),whose average recoveries were 93.34％-105.64％ with the RSDs of 0.5％-2.6％.CONCLUSION This accurate,reliable,sensitive and reproducible method can be used for the quality control of carbohydrates in Qingzhi Huayu Formula.

关键词

清脂化瘀方/HPLC-CAD特征图谱/糖类成分/质量控制

Key words

Qingzhi Huayu Formula/HPLC-CAD characteristic chromatograms/carbohydrate components/quality control

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出版年

2024

中成药

国家食品药品监督管理局,信息中心中成药信息站,上海中药行业协会

中成药

CSTPCD北大核心

影响因子：1.217

ISSN：1001-1528

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