Effects of different decocting methods on enzyme activities and main components during the preparation of Zhizichi Decoction
AIM To explore the effects of different decocting method on the enzyme activities and main components during the preparation of Zhizichi Decoction.METHODS During the processes of soaking and combined decoction,single decoction and decocting early of Sojae Semen Praeparatum,and decocting later of Sojae Semen Praeparatum,the activities of cellulase and β-glucosidase were detected,after which HPLC was adopted in the content determination of genipin 1-gentiobioside,geniposide,daidzein,genistein,crocin Ⅱ,and crocin Ⅰ,grey correlation analysis was used for investigating the correlations between enzyme activities and constituent contents.RESULTS With the prolongs of soaking time and decocting time,various main constituent contents demonstated increasing trends.The activities of cellulase and β-glucosidase were stable at the time of decocting later of Sojae Semen Praeparatum,whose correlation coefficients with constituent contents were only about 0.5.CONCLUSION The decocting later of Sojae Semen Praeparatum exhibits "glycoside protection by inactivating enzyme"effect to a certain extent,which can provide reference for research on the drug efficacy of Zhizichi Decoction.
万方数据
维普
