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茅苍术根茎、须根挥发性成分比较

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目的 比较茅苍术根茎、须根挥发性成分.方法 制作根茎、须根石蜡切片,光学显微镜下观察其横切面.水蒸气蒸馏法提取挥发油.GC-MS法检测苍术素、β-桉叶醇、茅术醇、苍术酮含量,并分析挥发性成分.采用多元统计分析软件进行主成分分析及差异代谢物筛选.结果 根茎横切面可见大型油室,须根中可见油细胞.须根挥发油含量占根茎的24.88%,共检测出共有成分55种、根茎特有成分2种、须根特有成分6种.根茎中β-桉叶醇、茅术醇、苍术酮、苍术素含量比例为22.84:1.16:2.78:1.00,须根中为5.59:0.22:0.97:1.00.根茎、须根中挥发性成分具有显著性差异,组间区分度良好.根据OPLS-DA模型筛选出茅术醇、β-桉叶醇等12种差异代谢物.结论 茅苍术根茎、须根挥发油成分类似,但含量不同,后者综合利用潜力更大.
Comparison of volatile constituents from the rhizomes and fibrous roots of Atractylodes lancea
AIM To compare the volatile constituents from the rhizomes and fibrous roots of Atractylodes lancea (Thunb.) DC..METHODS Paraffin sections of rhizomes and fibrous roots were made,and their transverse sections were observed under an optical microscope.Volatile oil was extracted by hydrodistillation.The contents of atractylodin,β-eudesmol,hinesol and atractyline were detected by GC-MS,and the volatile constituents were analysed.Principal component analysis and differential metabolite screening were performed by multivariate statistical analysis software.RESULTS In the transverse section of rhizomes,large oil chambers were observed,while oil cells were found in the fibrous roots.The volatile oil content in the fibrous roots is 24.88% of that in the rhizomes.A total of 55 common components,2 unique components of rhizomes and 6 unique components of fibrous roots were detected.The ratio of β-eudesmol,hinesol,atractyline and atractylodin in the rhizomes was 22.84∶1.16∶2.78∶1.00,while that in the fibrous roots was 5.59∶0.22∶0.97∶1.00.The volatile constituents in the rhizomes were significantly different from those in the fibrous root,and the groups were well discriminated from each other.Based on the OPLS-DA model,12 differential metabolites including hinesol and β-eudesmol were screened.CONCLUSION The volatile constituents of the rhizomes and fibrous roots of A.lancea are similar,but the contents are different,and the latter has a greater potential for comprehensive utilization.

Atractylodes lancea (Thunb.) DC.rhizomesfibrous rootsvolatile constituentsGC-MS

陈袁袁、陈雷、王萌、孙琳棋、邓娟、黄晓、陈庆梅、卫威、万雨露、汪文杰、余坤

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湖北中医药大学药学院,湖北 武汉430065

湖北省中医院,湖北 武汉 430060

湖北宏图中药材科技有限公司,湖北 黄冈438000

茅苍术 根茎 须根 挥发性成分 GC-MS

2024

中成药
国家食品药品监督管理局,信息中心中成药信息站,上海中药行业协会

中成药

CSTPCD北大核心
影响因子:1.217
ISSN:1001-1528
年,卷(期):2024.46(10)