AIM To optimize the stir-frying with sand process for Aconitum brachypodum Diels.,and to investigate its acute toxicity,anti-arrhythmic activity.METHODS With frying temperature,frying time and drug-sand ratio as influencing factors,comprehensive score for total contents of diester alkaloids (aconitine,yunaconitine) and monoester alkaloids (benzoylaconitine,16-epi-pyroaconitine,8-deacetylyunaconitine,pyroyunaconitine) as evaluation indices,the stir-frying with sand process was optimized by Box-Behnken response surface method.Acute toxicity test was adopted in the calculation of LD50 of raw product and stir-frying with sand-processed product.The model for rat arrhythmia was established,after which ventricular premature beat latency and incidence of ventricular tachycardia were determined.RESULTS The optimal conditions were determined to be 210℃ for frying temperature,15 min for frying time was,and 1∶15 for drug-sand ratio,the comprehensive score was 93.932.The LD50 of raw product,stir-frying with sand-processed product were 0.076,0.799 g/kg,respectively.Compared with the model group,different doses (5,8,12,16 mg/kg) of stir-frying with sand-processed product groups demonstrated prolonged ventricular premature beat latencies (P<0.05).Compared with the 5 mg/kg dose group,the 12-16 mg/kg dose group displayed decreased incidence of ventricular tachycardia (P<0.05).CONCLUSION Reduced acute toxicity of A.brachypodum is observable after stir-frying with sand,which exhibits strong anti-arrhythmic activity at high dose.
维普
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