Effects of dried extract powder of Qingre Xiaoyanning on a mouse model of rhinovirus infection
AIM To investigate the effects of dried extract powder of Qingre Xiaoyanning on a mouse model of rhinovirus (RhV) infection.METHODS Ninety-six BALB/c mice,half males and half females,were randomly divided into the normal group,the model group,the ribavirin group (60 mg/kg) and the low,medium and high dose groups of dried extract powder of Qingre Xiaoyanning (8.72,17.44,34.89 g/kg) for the administration of the corresponding doses of drugs once daily for 7 days.Except for those of the normal group,the mice of the other groups were intranasally instilled with VR-1187 RhV on the fourth day of the administration.On the second day to the day of the last administration,the mice had their left bronchus and left lung procured for pathological examination;their right lung taken for PCR detection of viral load;their levels of inflammatory factors TNF-α,IL-1β,IL-4,VCAM-1 and IFN-γ in alveolar lavage fluid detected by ELISA;their levels of macrophages,NK cells and lymphocytes detected by flow cytometry;and their pulmonary protein expressions of TLR4,MYD88,IKK-β,IκB and p-IκB detected by Western blot.RESULTS Compared with the model group,the group intervened with high dose dried extract powder of Qingre Xiaoyanning displayed decreased viral load (P<0.01);decreased levels of IL-4,VCAM-1 and IL-1β in bronchoalveolar lavage fluid (P<0.05,P<0.01);decreased proportion of pulmonary macrophages (P<0.01);increased proportion of lymphocytes and NK cells (P<0.05);and decreased protein expressions of TLR4,MYD88,IκB,p-IκB and ratio of p-IκB/IκB in lung tissue (P<0.05).Additionally,the medium dose group of dried extract powder of Qingre Xiaoyanning shared decreased pulmonary IKK-β protein expression (P<0.05).CONCLUSION The dried extract powder of Qingre Xiaoyanning can reduce the inflammatory reaction in mice to protect their lung tissue because of its obvious anti-RhV effect in vivo by inhibiting the RhV replication via TLR4/MYD88/NF-κB signaling pathway.
万方数据
维普
