Chemical constituents from Jiannaoning Mixture and their antioxidant activities
AIM To study the chemical constituents from Jiannaoning Mixture and their antioxidant activities.METHODS The extract was isolated and purified by silica gel,ODS,MCI,Sephadex LH-20 and semi-preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The antioxidant activities were studied by superoxide dismutase activity test.RESULTS Twenty compounds were isolated and identified as erythro-1-(4-hydroxy-3-methoxyphenyl)-2-{4-[(E)-3-n-butetheryl-1-propenyl]-2-methoxyphenoxy}-1,3-propanediol (1),threo-1-(4-hydroxy-3-methoxyphenyl)-2-{4-[(E)-3-n-butetheryl-1-propenyl]-2-methoxyphenoxy}-1,3-propanediol (2),5-hydroxy methylfurfural (3),syringin (4),benzyl-β-D-glucopyranoside (5),spinosyn (6),cordifolioidyne A (7),adenosine (8),jujubosides A (9),jujubosides B (10),salvianolic acid B (11),methyl 3,4-dihydroxy-phenyl lactate (12),methylophiopogonanone A (13),methylophiopogonanone B (14),tanshinone ⅡA (15),ethylparaben (16),triallyl isocyanurate (17),betulinic acid (18),diosgenin (19),4-(9H-β-carbolin-1-yl)-4-oxobut-2-enoic acid methyl ester (20).The IC50 values of compounds 7,11 and 19 were (8.52±1.13),(17.10±2.89),(11.45±1.31) μmol/L,respectively.CONCLUSION Compounds1-2 are new neolignans.Compounds3-5,8,16-17,20 are first isolated from the single ingredient of this formula.Compounds 7,11,17 have antioxidant activities.
Jiannaoning Mixturechemical constituentsisolation and identificationantioxidant activitestraditional Chinese medicine compound
万方数据
维普
