摘要
目的 研究健脑宁合剂的化学成分及其抗氧化活性.方法 提取物采用硅胶、ODS、MCI、Sephadex LH-20及半制备型HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构.采用类超氧化物歧化酶活性测试法进行抗氧化活性研究.结果 从中分离得到20个化合物,分别鉴定为赤式-1-(4-羟基-3-甲氧基苯基)-2-{4-[(E)-3-正丁醚基-1-丙烯基]-2-甲氧基苯酚基}-1,3-丙二醇(1)、苏式-1-(4-羟基-3-甲氧基苯基)-2-{4-[(E)-3-正丁醚基-1-丙烯基]-2-甲氧基苯酚基}-1,3-丙二醇(2)、5-羟甲基糠醛(3)、紫丁香苷(4)、benzyl-β-D-glucopyranoside(5)、斯皮诺素(6)、cordifolioidyne A(7)、腺苷(8)、酸枣仁皂苷A(9)、酸枣仁皂苷B(10)、丹酚酸B(11)、丹参素甲酯(12)、甲基麦冬黄烷酮A(13)、甲基麦冬黄烷酮B(14)、丹参酮ⅡA(15)、羟苯乙酯(16)、三烯丙基异氰脲酸酯(17)、白桦酯酸(18)、薯蓣皂苷元(19)、4-(9H-β-carbolin-1-yl)-4-oxobut-2-enoic acid methyl ester(20).化合物7、11、19的IC50值分别为(8.52±1.13)、(17.10±2.89)、(11.45±1.31)μmol/L.结论 化合物1～2为新木脂素类化合物,化合物3～5、8、16～17、20为首次从该组方单味药中分离得到.化合物7、11、19具有抗氧化活性.
Abstract
AIM To study the chemical constituents from Jiannaoning Mixture and their antioxidant activities.METHODS The extract was isolated and purified by silica gel,ODS,MCI,Sephadex LH-20 and semi-preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The antioxidant activities were studied by superoxide dismutase activity test.RESULTS Twenty compounds were isolated and identified as erythro-1-(4-hydroxy-3-methoxyphenyl)-2-{4-[(E)-3-n-butetheryl-1-propenyl]-2-methoxyphenoxy}-1,3-propanediol (1),threo-1-(4-hydroxy-3-methoxyphenyl)-2-{4-[(E)-3-n-butetheryl-1-propenyl]-2-methoxyphenoxy}-1,3-propanediol (2),5-hydroxy methylfurfural (3),syringin (4),benzyl-β-D-glucopyranoside (5),spinosyn (6),cordifolioidyne A (7),adenosine (8),jujubosides A (9),jujubosides B (10),salvianolic acid B (11),methyl 3,4-dihydroxy-phenyl lactate (12),methylophiopogonanone A (13),methylophiopogonanone B (14),tanshinone ⅡA (15),ethylparaben (16),triallyl isocyanurate (17),betulinic acid (18),diosgenin (19),4-(9H-β-carbolin-1-yl)-4-oxobut-2-enoic acid methyl ester (20).The IC50 values of compounds 7,11 and 19 were (8.52±1.13),(17.10±2.89),(11.45±1.31) μmol/L,respectively.CONCLUSION Compounds1-2 are new neolignans.Compounds3-5,8,16-17,20 are first isolated from the single ingredient of this formula.Compounds 7,11,17 have antioxidant activities.
维普
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