摘要
目的 研究酸枣叶的化学成分及其体外抗炎活性.方法 酸枣叶70％乙醇提取物采用多种现代色谱学方法进行分离纯化,根据理化性质及波谱数据鉴定所得化合物结构.采用RAW264.7模型评价其体外抗炎活性.结果 从中分离得到14个化合物,分别鉴定为ampelopsisionoside(1)、(3S,6S)-cis-linalool-3,7-oxide-β-D-glucopyranoside(2)、alangionoside L(3)、2-{(2S,5R)-5-[(1E)-4-hydroxy-4-methylhexa-1,5-dien-1-yl]-5-methyltetra-hydrofuran-2-yl}propan-2-ylβ-D-glucopyranoside(4)、(-)-甘密树皮素B-β-D-吡喃葡萄糖苷(5)、nikoenoside(6)、kiwiionoside(7)、dendranthemoside A(8)、委陵菜酸(9)、2α,3β,19α-三羟基乌苏-12-烯-28-酸(10)、2α-羟基白桦脂酸(11)、2α-羟基乌索酸(12)、ceanothanolic acid(13)、山楂酸(14).化合物10、11、13和14可抑制RAW264.7细胞生成NO.结论 化合物1、2和4为首次从鼠李科分离得到,化合物3、5、6和8为首次从该植物中分离得到.化合物10、11、13和14具有较强的抗炎活性.
Abstract
AIM To study the chemical constituents from the leaves of Ziziphus jujuba Mill.var.spinosa (Bunge) Hu ex H.F.Chou and their in vitro anti-inflammatory activities.METHODS The 70％ ethanol extract from the leaves of Z.jujuba var.spinosa was isolated and purified by various modern chromatographic methods,then the structures of obtained compounds were identified by physicochemical properties and spectral data.Their in vitro anti-inflammatory activities were evaluated by RAW264.7 model.RESULTS Fourteen compounds were isolated and identified as ampelopsisionoside (1),(3S,6S)-cis-linalool-3,7-oxide-β-D-glucopyranoside (2),alangionoside L (3),2-{(2S,5R)-5-[(1E)-4-hydroxy-4-methylhexa-1,5-dien-1-yl]-5-methyltetra-hydrofuran-2-yl} propan-2-yl β-D-glucopyranoside (4),(-)-nectandrin B-β-D-glucopyranoside (5),nikoenoside (6),kiwiionoside (7),dendranthemoside A (8),tormentic acid (9),2α,3β,19α-trihydroxy-urs-12-en-28 oic acid (10),2α-hydroxybetulinic acid (11),2α-hydroxylursolic acid (12),ceanothanolic acid (13),and maslinic acid (14).Compounds 10,11,13 and 14 could inhibit the production of NO in RAW264.7 cells.CONCLUSION Compounds 1,2,4 are first isolated from Rhamnaceae family,and 3,5,6,8 are first isolated from this plant.Compounds 10,11,13 and 14 have significant anti-inflammatory activities.
维普
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