首页|巴西产酒神菊正己烷部位化学成分及其体外抗肝损伤活性研究

巴西产酒神菊正己烷部位化学成分及其体外抗肝损伤活性研究

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目的 研究巴西产酒神菊正己烷部位的化学成分及其体外抗肝损伤作用.方法 采用GC-MS和LC-MS鉴定化学成分其及入血成分.采用乙醇诱导的LO2细胞评价其抗肝损伤作用,并检测细胞存活率和细胞ROS、ALT、AST、GSH、GST水平.结果 从正己烷部位中分离鉴定出喇叭茶醇(8.55%)、绿花白千层醇(6.83%)、桉油烯醇(4.26%)等54个化合物,含药血清中鉴定出石竹素、(-)-α-荜澄茄油烯等7个原型成分.与模型组组比较,含药血清组的细胞存活率明显升高(P<0.01),ALT、AST、ROS水平显著下降(P<0.01),GST、GSH水平显著升高(P<0.05).结论 巴西产酒神菊正己烷部位具有体外具有一定的抗氧化活性和保肝作用,发挥抗氧化活性作用的可能是倍半萜类物质.
Chemical constituents from the n-hexane fraction of Baccharis trimera from Brazil and their in vitro anti-liver injury activities
AIM To study the chemical constituents from the n-hexane fraction of Baccharis trimera (Less.) DC from Brazil and their in vitro anti-liver injury activities.METHODS The chemical constituents and blood components were identified by GC-MS and LC-MS.The ethanol-induced LO2 cells were used to evaluate their anti-liver injury effects,and the cell survival rate and the levels of ROS,ALT,AST,GSH and GST were detected.RESULTS A total of54 compounds were isolated and identified from n-hexane fraction,including (-)-palustrol (8.55%),viridiflorol (6.83%),spathulenol (4.26%),and 7 prototype components such as caryophyllin and (-)-α-cubebene were identified from the drug-containing serum.Compared with the model group,the cell viability rate of drug-containing serum group was significantly increased (P<0.01),and the levels of ALT,AST and ROS were significantly decreased (P<0.01),and the levels of GST and GSH were significantly increased (P<0.05).CONCLUSION The n-hexane fraction from the Baccharis trimera has a certain in vitro antioxidant activity and hepatoprotective effects.It' s possible that sesquiterpenoids play a role in antioxidant activity.

Baccharis trimera (Less.) DCchemical constituentsGC-MSdrug-containing serumLO2 cellsantioxidant activitiesanti-liver injury

杨雄、刘宏栋、蒋涛、闵建新、李斌

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江西中医药大学院士工作站,江西 南昌330004

酒神菊 化学成分 GC-MS 含药血清 LO2细胞 抗氧化活性 抗肝损伤

2024

中成药
国家食品药品监督管理局,信息中心中成药信息站,上海中药行业协会

中成药

CSTPCD北大核心
影响因子:1.217
ISSN:1001-1528
年,卷(期):2024.46(12)