Distribution and prophage analysis of Candidatus Liberibacter asiaticus in Leibo navel orange
In order to understand the titer difference and genetic diversity of Candidatus Liberibacter asiaticus(CLas)in different tissue of Leibo navel orange,and provide theoretical foundation for the epidemiology of Citrus Huanglongbing in Leibo County,the Real-time PCR was used to analyze the absolute quantitation of CLas in eight tissues(leaf of new shoot,phloem of new shoot,fruit pedicle,fruit pith,fruit central axis,leaf of branch,phloem of upper branch,phloem of lower branch)and different segments of pith of Leibo navel orange,and to identify the prophage type.The results showed that the content of CLas in fruit tissues(pedicle,pith,central axis)was significantly higher than that in shoot tissue and branch tissue.The content of CLas in pith was asymmetrical and concentrated in the middle part of fruit.The prophage type identification showed that CLas was Type 2+Type 4 in the new shoot tissue and Type 1 in the other mature tissue,and there was mixed infection of the two types of CLas in the fruit pedicle.In conclusion,CLas was mainly concentrated in the middle pith of fruit tissue in Leibo navel orange,and at present,there was a situation of external CLas infecting shoot tissue in Leibo County.
Leibo navel orangeCandidatus Liberibacter asiaticusdistribution patternprophage type
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