摘要
以10年生烟富3/M26/八棱海棠(Malus robusta)为试材,运用13C同位素示踪技术,在果实膨大期叶面喷施浓度分别为50、100、150、200 mg/L的黄腐酸水溶液,以喷施清水为对照,研究了叶片光合参数、同化能力、光合产物的输出和自留比例及光合产物分配特性的变化.结果 表明:与其他处理相比,喷施150 mg/L的黄腐酸水溶液,叶片光合性能最优,山梨醇和蔗糖含量、6-磷酸山梨醇脱氢酶(S6PDH)和蔗糖磷酸合酶(SPS)活性最高;同时,13C示踪结果表明,喷施150 mg/L黄腐酸水溶液显著增强了叶片同化能力,提高了光合产物的输出比率,降低了叶片的自留比率,增加了果实13C的积累量,提高了果实成熟期果实糖含量.综上,叶片喷施150 mg/L黄腐酸水溶液可以增强叶片的光合作用,提高叶片光合产物的合成能力,促进叶片中光合产物的输出比率,有利于果实光合产物的积累.
Abstract
Using 10-year-old Yanfu 3/M26/Malus robusta as the experimental material,13C isotope tracer technology was applied to foliar spray of different concentrations of fulvic acid(50 mg/L,100 mg/L,150 mg/L,200 mg/L,expressed by FA50,FA100,FA150,FA200,respectively)during the fruit expansion period,and spraying of water as the control,to study the photosynthetic parameters,assimilation capacity,photosynthetic product output and self-retention ratio,and photosynthetic product distribution characteristics in fruits and leaves.The results showed that the treatment of foliar application of 150 mg/L fulvic acid had the best performance in leaf photosynthetic performance,sorbitol and sucrose content,sorbitol 6-phosphate dehydrogenase(S6PDH)and sucrose phosphate synthase(SPS)activities compared with other treatments.Meanwhile,13C tracing results showed that the treatment of foliar application of 150 mg/L fulvic acid significantly enhanced leaves assimilation capability,increased the proportion of photosynthetic products exported,reduced the proportion of leaves self-retention,and increased fruit 13C accumulation.In conclusion,the treatment of foliar application of 150 mg/L fulvic acid on leaves enhanced photosynthesis,improved the synthesis capacity of photosynthetic products in leaves,promoted the output ratio of photosynthetic products in leaves,and facilitated the accumulation of photosynthetic products in fruits.
基金项目
山东省重点研发计划(2021CXGC010802)
山东省重点研发计划(2022TZXD008)
财政部和农业农村部:国家现代农业产业技术体系建设专项(CARS-27)
国家科技期刊平台
NSTL
万方数据