Cloning and sequence analysis of self-incompatibility S-RNase genes from Prunus humilis germplasm resources
The objective of this study was to identify the S genotypes of different Prunus humilis germplasm resources and analyze the sequence characteristics of the Prunus humilis S gene.A total of 70 Prunus humilis germplasm resources were used as test materials.The S gene was amplified through PCR,followed by cloning and sequencing to obtain its gene sequence.Using the BFP93/BFP94-1 primers,two S alleles were identified in each of the 52 germplasm resources.Single S allele was identified in 16 germplasm resources.No bands were amplified in the'3-32-bianhuang'and'10-32'germplasm resources.A total of 120 genes were cloned,and 36 S genotypes were identified.The new genes were registered in NCBI with registration numbers in the following sequence OQ124075～OQ124109.
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