Identification and biological characteristics analysis of one pathogen causing pomegranate dry rot in Henan
In October 2020,in pomegranate disease investigation in Shangqiu Henan Province,it was found that the pomegranate fruits had a disease of dry rot.The lesions on the pomegranate were lost water and shrank,becoming dry,hard and black-brown patches.The whole fruit was dry in a serious state.The diseased pomegranate fruits were picked,separated and purified by tissue separation method to obtain 5 pathogenic strains with same morphological characteristics,and a selected strain SQPGBD1 was used as test strain.The pathogen was identification as Botryosphaeria dothidea.According to morphological characteristic,pathogenicity identification and multi-gene(ITS,TUB2 and EF1-α)phylogenetic analysis.The biological characteristics of the pathogen SQPGBD1 were determined according to the cross method.The results showed that the most suitable pH value for the strain was 6,the temperature was 30℃,and the light conditions were 12 hours of light and 12 hours of darkness.The most favorable carbon source condition for the growth of SQPGBD1 strain was glucose,and the nitrogen source condition was sodium nitrate.The experimental results of this study have created an theoretical basis for the comprehensive control of pomegranate dry rot.
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