不同红蓝光配比对樱桃幼苗形态发育、抗坏血酸和活性氧代谢的影响
Effects of different proportions of red and blue light on morphological development and metabolism of ascorbic acid and reactive oxygen species in cherry seedlings
胡举伟 1郑磊 2孟鑫 3朱晓洁 4宋涛 1李新柱1
作者信息
- 1. 金正大生态工程集团股份有限公司,养分资源高效利用全国重点实验室,农业农村部植物营养与新型肥料创制重点实验室,山东临沂 276700
- 2. 广东清远金正大农业研究院有限公司
- 3. 沃夫特复合肥有限公司
- 4. 河南豫邮金大地科技服务有限公司
- 折叠
摘要
为了探索樱桃幼苗发育、抗氧化酶活性、抗坏血酸和活性氧代谢对红蓝组合光的响应机制,采用LEDs为光源,以鲁樱3号樱桃幼苗为试验材料,设红光(0％B)、蓝光(100％B)和红蓝组合光(15％B、20％B、30％B、50％B)6个处理,以白光为对照,研究了不同红蓝光配比处理对樱桃幼苗形态发育、总抗坏血酸库、抗坏血酸代谢相关酶活性、活性氧积累和活性氧清除能力的影响.结果表明,红光可促进樱桃幼苗的茎伸长和叶片展开,红光、蓝光和红蓝组合光处理樱桃幼苗叶片抗坏血酸含量、脱氢抗坏血酸含量以及总抗坏血酸库、抗氧化酶活性均明显高于白光对照.蓝光比率(15％～50％)增大更利于樱桃叶片抗氧化能力提高,减轻膜脂过氧化损伤,但蓝光(100％B)处理引起樱桃叶片活性氧积累和丙二醛含量相对增加,不利于樱桃幼苗叶片生理代谢稳定.
Abstract
In order to explore the mechanism of response of development and antioxidant enzymes activity and metabolism of ascorbic acid and reactive oxygen species in cherry seedlings to the combination of red and blue light.The LEDs as light sources were used in this study with 6 treatments including red light(0％B),blue light(100％B)and combinations of red and blue light(15％B,20％B,30％B,50％B),white light as control,and the effects of different proportions of red and blue light on the development,total ascorbate pool,ascorbic acid metabolism-related enzymes,accumulation of reactive oxygen species and scavenging ability of reactive oxygen species in cherry seedlings('Luying 3')were studied.The results showed that red light could promote stem elongation and leaf expansion of cherry seedlings,the ascorbic acid content,dehydroascorbic acid content,total ascorbate pool and antioxidant enzymes activity of cherry leaves under the treatments of red light,blue light and the combination of red and blue light were higher than cherry leaves under the treatment of white light control.The increase of blue light percentage(15％～50％)was more beneficial to the increase of antioxidant capacity of cherry leaves,and reduced peroxidation damage of ROS to membrane lipids,but the blue light(100％B)caused relative increase of accumulation of ROS and malondialdehyde content in cherry leaves.The blue light(100％B)was not conducive to the stability of physiological metabolism of cherry seedling leaves.
关键词
樱桃/光质/抗坏血酸/抗氧化酶/活性氧Key words
fruiting cherry/light quality/ascorbic acid/antioxidant enzyme/reactive oxygen species引用本文复制引用
基金项目
国家重点研发计划(2022YFD1700605)
山东省重点研发计划(2022SFGC0301)
驻马店市科技重大专项()
出版年
2024
国家科技期刊平台
NSTL
万方数据