摘要
对内蒙古地区主栽的4个葡萄品种的枝条及根系进行抗寒性分析,旨在为葡萄抗寒育种、优良品种推广提供理论依据.以北玫、双庆、玫瑰香和芳堤娜为试验材料,模拟自然冷冻降温过程,枝条模拟温度设置为-5、-15、-20、-25、-30、-35、-40℃;根系模拟温度设置为 0、-3、-6、-9、-12、-15、-18℃,分别测定丙二醛含量、可溶性糖含量、可溶性蛋白含量、脯氨酸含量.结果表明,随着温度逐渐降低,4个葡萄品种的渗透调节物质均呈现显著差异.其中,根系可溶性蛋白含量均呈上升趋势,枝条可溶性蛋白含量均呈先上升后下降的趋势,枝条和根系的丙二醛含量和脯氨酸含量均呈上升趋势,而可溶性糖含量均呈先上升后下降的趋势.通过隶属函数法分析,4个葡萄品种的抗寒性强弱顺序为双庆＞北玫＞芳堤娜＞玫瑰香.
Abstract
In this study,the cold resistance of branches and roots of four grape varieties mainly cultivated in Inner Mongolia was analyzed,aiming to provide a theoretical basis for cold resistance breeding and promotion of grape varieties.In this experiment,'Beimei','Shuangqing','Midknight Beauty'and'Frontenac'were used as test materials to simulate the natural freezing cooling process,and the simulated temperature of shoots was set at-5 ℃,-15 ℃,-20 ℃i,-25 ℃,-30 ℃,-35 ℃,-40 ℃.The simulated temperature of the root system was set at 0 ℃,-3 ℃,-6 ℃,-9 ℃,-12 ℃,-15 ℃ and-18 ℃,and the content of malondialdehyde,soluble sugar,soluble protein and proline were determined,respectively.The results showed that with the gradual decrease of temperature,the osmotic regulators of four grape varieties showed significant differences.Among them,the soluble protein content of roots showed an upward trend,the soluble protein content of shoots increased first and then decreased,the malondialdehyde content and proline content of branches and roots showed an upward trend,and the soluble sugar content increased first and then decreased.According to the membership function analysis,the cold resistance of four grape varieties was as follows:'Shuangqing'＞'Beimei'＞'Frontenac'＞'Midknight Beauty'.
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