Virus detection and analysis of infection types of pitaya in Guizhou
To clarify the types and mixed infection types of Guizhou pitaya viruses,as well as the dynamics of virus occurrence in the main production areas.A total of 703 pitaya stems were collected from pitaya orchards in Luodian County,Wangmo County,Zhenfeng County,and Zhenning County,Guizhou Province.Suspected viral disease samples were detected by RT-PCR using CVX,CVX-NTU,PiVX,ZyVX,and SchVX specific primers.The detection rates of pitaya virus infection in Luodian in 2021 and 2022 were 85.92％and 94.90％,Wangmo was 93.48％and 93.90％,Zhenfeng was 90.99％and 99.02％,and Zhenning was 94.52％and 94.17％;Among which,in 2021,SchVX was not detected in Wangmo,and in 2022,there was a high detection rate(both of which were mixed infection),indicating the spread of SchVX in Wangmo.In 2021 and 2022,the mixed infection detection rates of pitaya virus were 83.10％and 90.82％in Luodian,93.48％and 87.80％in Wangmo,90.09％and 99.02％in Zhenfeng,and 93.15％and 93.34％in Zhenning.From the detection perspective of mixed infection types,9 types were detected of 2 viruses mixed infection,10 types of 3 viruses mixed infection were detected,5 types of 4 viruses mixed infection were detected,and the type of 5 virus mixed infection.The virus infection of Guizhou pitaya is mainly caused by a combination of several PVX viruses,and has occurred extensively in the main pitaya production areas of Guizhou.
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万方数据
