Development and evaluation of rapid visualization detection method for Xanthomonas fragariae based on RPA-LF
The objective of this study was to establish a stable,rapid,and accurate technical method for the detection of Xanthomonas fragariae in the field.Achieving early diagnosis and effective control of Xanthomonas fragariae.This study established an RPA-LF assay for Xanthomonas fragariae.We designed RPA-specific primers and probes in the conserved region of the restriction endonuclease subunit S gene sequence of Xanthomonas fragariae.And we also optimized the reaction conditions of the method,analyzed its sensitivity and specificity.Finally,this study detected suspicious samples performed.The optimal reaction conditions were 39℃for 10 min.The minimum detection limit was 1.0 × 101 copies/μL.The method was able to detect Xanthomonas fragariae specifically.It took only 30 min from the collection of the suspect plant to the detection of Xanthomonas fragariae.The RPA-LF assay for Xanthomonas fragariae established in this study is rapid,simple,accurate.Eventually,we can visualize this pathogen for early diagnosis.
NETL
NSTL
万方数据
