Establishment of CX5 tissue culture system of apple rootstock
Stem segments of dormant branches and stem segments of new branches from late April to early June of apple semi-dwarfing anvils were used as experimental materials.In vitro tissue culture method was used to study the types of explants suitable for rapid propagation of CX5 tissue culture,sterilization method,sterilization time,and the effects of different plant growth regulators on proliferation and culture.The results showed that CX5 explants suitable for sterilization were stem segments of new shoots,and their browning and contamination rates were relatively low.The sterilization time of 0.1％HgCl2 for explants was controlled to about 6 min,and the damage was minimal.CX5 stem axillary bud induction medium was MS+0.75 mg/L 6-BA+0.3 mg/L IBA,suitable for tissue culture seedlings proliferation and subculture medium was MS+0.7 mg/L 6-BA+0.5 mg/L IBA,rooting medium was 1/2 MS+0.2 mg/L IAA.The CX5 tissue culture and rapid propagation technology system was established.
apple semi-dwarfing anvilCX5stem segmentstissue culture
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