Optimization of tissue culture conditions of 16 dwarf banana varieties(lines)
The cultivation and promotion of dwarf banana varieties(lines)are helpful to reduce typhoon losses,reduce labor and material costs,and improve industrial efficiency.A number of dwarf banana varieties(lines)have been screened and cultivated in the early stage,which have the potential for promotion,but the efficiency of tissue culture and rapid propagation is very low.Therefore,the screening and optimization of tissue culture technology of dwarf banana varieties(lines)are carried out to promote the application of dwarf banana varieties(lines).The results showed that the level of plant growth regulators for initiating proliferation,sub-culture and rooting culture of dwarf banana varieties(lines)was lower than that of conventional control variety'Baxijiao'.The optimum combination of plant growth regulators for'Baxijiao'were 4.0 mg/L 6-BA+0.1 mg/L NAA(initiating proliferation),3.0 mg/L 6-BA+0.2 mg/L NAA(sub-culture),0.3 mg/L NAA(rooting culture),while the optimum combination of plant growth regulators for dwarf banana varieties(lines)were 1.0～2.0 mg/L 6-BA+0.05～0.1 mg/L NAA(initiating proliferation),1.0～2.0 mg/L 6-BA+0.05～0.1 mg/L NAA(sub-culture),0.1～0.2 mg/L NAA(rooting culture),the induction rate,proliferation rate,effective bud rate and root development were close to'Baxijiao'.The research results will be helpful to the production of tissue culture seedlings of dwarf banana varieties(lines),the development and application of exogenous plant growth regulators regulation technology,and the promotion of dwarf banana varieties.
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