新疆苹果褐色斑点果腐病病原菌的鉴定
Identification of the pathogen of apple brown spot fruit rot in Xinjiang
苏宣乐 1常晓云 1张王斌1
作者信息
- 1. 塔里木大学,南疆农业有害生物综合治理兵团重点实验室,农业农村部阿拉尔作物有害生物科学观测实验站,新疆阿拉尔 843300
- 折叠
摘要
为明确新疆苹果褐色斑点果腐病病原菌的种类,对采自阿拉尔的苹果褐色斑点果腐病病果的病原菌种类进行了鉴定.利用组织分离法对病原菌进行分离纯化,获得菌株PGFL,采用柯赫氏法则进行致病性验证,结合形态学特征观察和ITS、RPB2、EF序列构建多基因联合系统发育树对病原菌的种类进行确定.菌株PGFL的人工培养菌落形态呈现灰色至暗青褐色,与链格孢属Alternaria形态观察比对结果高度相似,且接种离体果实后可发病.分子生物学分析菌株PGFL和菌株Alternaria alternata MAFF 239887及A.alternataJLUAF6聚为一簇,与Alternaria属A.alternata的序列相似度最高,且自展支持率为100％.研究确定引起新疆苹果褐色斑点果腐病病原菌为链格孢A.alternata.
Abstract
To clarify the types of pathogenic bacteria causing brown spotted apple fruit rot in Xinjiang,the types of pathogenic bacteria causing brown spotted apple fruit rot in apple fruits collected from the region were identified.The pathogenic bacteria were isolated and purified using tissue separation method,and their pathogenicity was verified using Koch's rule.Combined with morphological observation and ITS,RPB2,and EF sequences,a multi gene combined phylogenetic tree was constructed to determine the type of pathogenic bacteria.The morphology of the artificially cultivated colonies of strain PGFL showed a gray to dark turquoise brown color,which was highly similar to the morphological observation of Alternaria in the genus Alternaria.Moreover,after inoculation with isolated fruits,it can cause disease.Molecular biology analysis showed that strain PGFL,strain Alternaria alternata MAFF 239887,and A.alternata JLUAF6 clustered into a cluster.The sequence similarity between this strain and the Alternaria genus A.alternata was the highest,with a self expanding support rate of 100％.Research has determined that the pathogen causing the brown spotted fruit rot of Xinjiang apples is A.alternata.
关键词
菌落形态/分生孢子/ITS/RPB2/EF/Alternaria/alternataKey words
colony morphology/conidia/ITS/RPB2/EF/Alternaria alternata引用本文复制引用
基金项目
&&(2018AB038)
&&(1221015)
出版年
2024
国家科技期刊平台
NSTL
万方数据