Identification of the pathogen of apple brown spot fruit rot in Xinjiang
To clarify the types of pathogenic bacteria causing brown spotted apple fruit rot in Xinjiang,the types of pathogenic bacteria causing brown spotted apple fruit rot in apple fruits collected from the region were identified.The pathogenic bacteria were isolated and purified using tissue separation method,and their pathogenicity was verified using Koch's rule.Combined with morphological observation and ITS,RPB2,and EF sequences,a multi gene combined phylogenetic tree was constructed to determine the type of pathogenic bacteria.The morphology of the artificially cultivated colonies of strain PGFL showed a gray to dark turquoise brown color,which was highly similar to the morphological observation of Alternaria in the genus Alternaria.Moreover,after inoculation with isolated fruits,it can cause disease.Molecular biology analysis showed that strain PGFL,strain Alternaria alternata MAFF 239887,and A.alternata JLUAF6 clustered into a cluster.The sequence similarity between this strain and the Alternaria genus A.alternata was the highest,with a self expanding support rate of 100％.Research has determined that the pathogen causing the brown spotted fruit rot of Xinjiang apples is A.alternata.
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