Preliminary analysis of MdCAXs gene family in apple fruit and identification of calcium transport function
Bitter pit(BP)is a prevalent physiological disease with calcium deficiency in apple fruit,that significantly impacts its appearance quality and commercial value.However,the molecular mechanism of bitter pit remains unclear.The Ca2+/H+antiporter(CAXs)has been found to be associated with calcium deficiency in plants.In this study,we analyzed the physical and chemical properties of 12 MdCAXs gene family members,along with their protein sequences.We also conducted correlation analysis between water-soluble mineral element content,and calcium transport-related gene expressions.This article examined the calcium transport functions of MdCAX2,MdCAX5,and MdCAX11,as well as sMdCAX2,sMdCAX5,and sMdCAX11 with NRR removed.The study revealed that MdCAX5 and sMdCAX11 may play a role in transporting Ca2+and consequently contribute to the regulation of bitter pit disease occurrence and development.
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