荔枝转录因子LCMYB8的克隆与表达分析
Cloning and expression analysis of litchi transcription factor LcMYB8
石雨雨 1卿昊炜 2桂秋林 2黎明 3易晨歆 3黄川 4陈艳艳 4刘红枫 4郭慧勤 3彭宏祥 3丁峰 3张树伟3
作者信息
- 1. 广西农业科学院,广西作物遗传改良生物技术重点开放实验室,南宁 530007;广西大学农学院
- 2. 广西大学农学院
- 3. 广西农业科学院,广西作物遗传改良生物技术重点开放实验室,南宁 530007
- 4. 广西灵山县农业科学研究所
- 折叠
摘要
为探究LcMYB8转录因子在荔枝成花调控中的作用,以妃子笑荔枝为试验材料,克隆LcMYB8转录因子的序列全长,并对其进行生物信息学分析.通过实时荧光定量PCR技术,分析LcMYB8转录因子在不同器官、年周期以及低温处理中的时空表达模式.结果显示,LcMYB8转录因子全长1 749 bp,其中开放阅读框915 bp,共编码304个氨基酸.时空表达模式表明,LcMYB8转录因子可能在荔枝成花和果实发育期起着关键作用.研究结果旨在为荔枝优质新品种培育提供理论指导.
Abstract
To investigate the role of LcMYB8 transcription factor in the regulation of litchi flowering,this study used'Feizixiao'litchi as experimental material,cloned the full length of LcMYB8 transcription factor sequence,and performed bioinformatics analysis on it.In addition,real-time fluorescence quantitative PCR technology was used to analyze the spatiotemporal expression patterns of LcMYB8 transcription factor in different tissues and organs,annual cycles and low-temperature treatment.The results showed that the LcMYB8 transcription factor had a total length of 1 749 bp,with an open reading frame of 915 bp,encoding a total of 304 amino acids.The spatiotemporal expression patterns suggest that the LcMYB8 transcription factor may play a crucial role in the flowering and fruit development stages of litchi.This study aims to provide theoretical guidance for the cultivation of high-quality new varieties of litchi.
关键词
荔枝/LcMYB8转录因子/表达模式/成花调控Key words
litchi/LcMYB8 transcription factor/expression pattern/floral regulation引用本文复制引用
基金项目
国家自然科学基金(32060659)
广西自然科学基金(2024GXNSFFA010016)
广西自然科学基金(2022GXNSFDA080010)
广西自然科学基金(2022GXNSFAA035605)
广西农科院科技发展基金(2021YT124)
广西壮族自治区科技重大专项(桂科AA23023007)
出版年
2024
国家科技期刊平台
NSTL
万方数据