首页|荔枝转录因子LCMYB8的克隆与表达分析

荔枝转录因子LCMYB8的克隆与表达分析

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为探究LcMYB8转录因子在荔枝成花调控中的作用,以妃子笑荔枝为试验材料,克隆LcMYB8转录因子的序列全长,并对其进行生物信息学分析.通过实时荧光定量PCR技术,分析LcMYB8转录因子在不同器官、年周期以及低温处理中的时空表达模式.结果显示,LcMYB8转录因子全长1 749 bp,其中开放阅读框915 bp,共编码304个氨基酸.时空表达模式表明,LcMYB8转录因子可能在荔枝成花和果实发育期起着关键作用.研究结果旨在为荔枝优质新品种培育提供理论指导.
Cloning and expression analysis of litchi transcription factor LcMYB8
To investigate the role of LcMYB8 transcription factor in the regulation of litchi flowering,this study used'Feizixiao'litchi as experimental material,cloned the full length of LcMYB8 transcription factor sequence,and performed bioinformatics analysis on it.In addition,real-time fluorescence quantitative PCR technology was used to analyze the spatiotemporal expression patterns of LcMYB8 transcription factor in different tissues and organs,annual cycles and low-temperature treatment.The results showed that the LcMYB8 transcription factor had a total length of 1 749 bp,with an open reading frame of 915 bp,encoding a total of 304 amino acids.The spatiotemporal expression patterns suggest that the LcMYB8 transcription factor may play a crucial role in the flowering and fruit development stages of litchi.This study aims to provide theoretical guidance for the cultivation of high-quality new varieties of litchi.

litchiLcMYB8 transcription factorexpression patternfloral regulation

石雨雨、卿昊炜、桂秋林、黎明、易晨歆、黄川、陈艳艳、刘红枫、郭慧勤、彭宏祥、丁峰、张树伟

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广西农业科学院,广西作物遗传改良生物技术重点开放实验室,南宁 530007

广西大学农学院

广西灵山县农业科学研究所

荔枝 LcMYB8转录因子 表达模式 成花调控

国家自然科学基金广西自然科学基金广西自然科学基金广西自然科学基金广西农科院科技发展基金广西壮族自治区科技重大专项

320606592024GXNSFFA0100162022GXNSFDA0800102022GXNSFAA0356052021YT124桂科AA23023007

2024

10.16626/j.cnki.issn1000-8047.2024.06.011

中国果树
中国农业科学院果树研究所

中国果树

CSTPCD
影响因子：0.45
ISSN：1000-8047
年,卷(期)：2024.(6)