Grape powdery mildew is one of the most important fungal diseases in viticulture,and its pathogen is Erysiphe necator.In this study,we investigated whether the TUB2 gene could serve as a DNA barcoding differentiating Erysiphe necatorand its closely-related species.The results revealed that the TUB2 genes show distinct discrepancies between intra-and inter-specific genetic distances in Erysiphesepeices.The diversity of the TUB2genes can effectively distinguish Erysiphe necatorand its closely-related species via phylogenetic study.Therefore,the TUB2region could be recommended as the ideal candidate DNA barcoding for the diagnose of grape powdery mildew.