Bioinformatics analysis of TUB2gene for DNA barcoding of the causal pathogen of powdery mildew on grape
Grape powdery mildew is one of the most important fungal diseases in viticulture,and its pathogen is Erysiphe necator.In this study,we investigated whether the TUB2 gene could serve as a DNA barcoding differentiating Erysiphe necatorand its closely-related species.The results revealed that the TUB2 genes show distinct discrepancies between intra-and inter-specific genetic distances in Erysiphesepeices.The diversity of the TUB2genes can effectively distinguish Erysiphe necatorand its closely-related species via phylogenetic study.Therefore,the TUB2region could be recommended as the ideal candidate DNA barcoding for the diagnose of grape powdery mildew.