Study on embryo culture technique of'Zixiang Seedless'grape as female parent
In order to cultivate the new germplasm of seedless grape,the seedless grape variety'Zixiang Seedless'was used as the female parent,and hybridized with the seedless varieties'Flame Seedless','Thompson Seedless','Liming Seedless','Beauty Seedless'and'Crimson Seedless'respectively,with hybrid ovules and'Zixiang Seedless'natural hybrid ovules as experimental materials for embryo rescue test,explore the sampling period,paternal genotype,the influence of embryo development rate and germination rate.The results showed that the embryo development rate and embryo germination rate of the six crosses were highest at 55 days after pollination,that is,the optimal sampling period was 55 days after pollination.The embryo development rate(46.99％)and germination rate(32.53％)of the hybrid embryos with'Flame Seedless'as the male parent were higher than those of the other 4 hybrid combinations except'Zixiang Seedless'.During the ovule development stage,the hybrid had the highest embryo development rate in W1 medium(MS+0.3 g/L cysteine+60 g/L sucrose+7 g/L agar+3 g/L activated carbon).
万方数据
维普
