Preliminary study on the effects of intercropping perilla on microorganisms and enzyme activities in kiwifruit inter-root soil and fruit quality
This paper analyzed the effects of kiwifruit intercropping with perilla on the rhizosphere soil micro-bial community,soil nutrients,enzyme activity,and kiwifruit fruit quality.The aim was to explore the intercropping effect of perilla on kiwifruit and provide theoretical basis for further promoting and applying the kiwifruit understory intercropping with perilla model.This study mainly investigated the changes in soil microbial communities under intercropping conditions through metagenomic sequencing technology.The experiment devised with 5 treatments:M1201+112,500 plants/hm2(LH),M626+112,500 plants/hm2(ZH),M1201+75,000 plants/hm2(LL),M626+75,000 plants/hm2(ZL),and kiwifruit monoculture(CK).The organic matter and alkaline nitrogen content of LH and ZH treatment significantly decreased during the flowering stage of perilla,while the available phosphorus significantly decreased during the harvest stage of perilla and the available potassium content significantly increased.The bacteria in the rhizosphere soil of kiwifruit were dominant,with the main bacterial genera being Bradyrhizobium,Streptomyces,Pseudomonas,etc.,the main fungal genera being Abelia,Homo,Mus,etc.,all showed significant improvement in the intercropping group,with the highest abundance in LH treatment.Correlation analysis found that Bradyrizobiumwas a negative correlation between soil β-xylosidase activity and available phosphorus content,while Abelia was positively correlated with soil acid phosphatase activity and soil leucine aminopeptidase activity.Compared with CK,the differential genes between LH and ZH treatment were mainly enriched in the glycosphingolipids biosynthesis-ganglio series and amide biosynthetic process,which helped to increase plant resistance.The intercropping group significantly increased the content of reduced ascorbic acid,soluble solids,and soluble sugars in kiwifruit,while reducing the total acid content.In summary,intercropping kiwifruit with perilla changed the soil microbial community structure,such as significantly increasing the abundance of Bradyrhizobium,which helped to improve fruit quality.Promoting the perilla M1201+112,500 plants/hm2 planting model while also compensating for the soil nutrients taken away by planting perilla.
万方数据
维普
