Comparative study on the flower bud differentiation characteristics of three blueberry varieties
In order to investigate the morphological changes,flower bud differentiation,flower bud differentiation rules and differences among varieties of Yunnan blueberry,the phenological phase of'Bluecrop','Sweetheart'and'Sharpblue'blueberry cultivars was observed and the flower bud differentiation process was analyzed by improved paraffin section method.MRM method was used to determine the content of 5 kinds of endogenous hormones in'Sharpblue'during 3 times of flower bud differentiation.The results showed that'Bluecrop'buds,blossoms and fruits once a year.'Sweetheart'appears buds,flowers and fruits twice a year,denoted as the first'Sweetheart'and the second'Sweetheart'respectively.'Sharpblue'blooms and fruits three times a year,denoted as the first'Sharpblue',the second'Sharpblue'and the third'Sharpblue',respectively.The time interval between the bud stage and the end of fruit ripening was significant between the first'Sweetheart'and the second'Sweetheart'.From bud to fruit ripening stage,the first'Sharpblue'and the second'Sharpblue'had the same growth period,and the second'Sharpblue'and the third'Sharpblue'also had the same growth period.There were six main stages of differentiation.The first stage was the beginning stage of differentiation,followed by the differentiation stage of inflorescence primordium,sepal primordium and petal primordium,and finally the differentiation stage of stamen primordium and carpel primordium.The differentiation time of the three blueberry varieties was different.'Bluecrop'took the longest time to complete flower bud differentiation(53 days),followed by'Sweetheart',which had longer differentiation time than'Sharpblue'.Under the same environmental conditions,there was a significant time interval between the two growth periods of'Sweetheart',and the degree of overlap of'Sharpblue'in these growth periods was different,and the time dividing line was not significant.Unlike'Bluecrop'and'Sweetheart'require low temperature induction,'Sharpblue'did not need to be induced in Yunnan,and'Sharpblue'entered flower bud differentiation immediately after spring self-cutting.Blueberry flower buds at the gestation stage required lower content of IAA,CKs and GAs,but higher content of ABA and ACC.In order to benefit the floral organs of flower bud differentiation at the later stage,IAA,CKs and GAs should be maintained in a balanced state.