60Co-γ射线辐照对石榴种子萌发及幼苗生长的影响

Effects of 60Co-γ irradiation on seed germination and seedling growth of pomegranate

高小峰 ¹鲁振华 ²王虹 ¹源朝政 ¹周晓静 ¹贾毛毛 ¹郭双双 ¹黄冉涛 ¹李玉英³

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作者信息

1. 南阳市科学院,河南省石榴种质资源创新与利用工程技术研究中心,南阳市石榴新品种选育重点实验室,473083
2. 中国农业科学院郑州果树研究所
3. 南阳师范学院
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摘要

以突尼斯软籽石榴种子为材料,采用 100、200、300、400 Gy的 60Co-γ射线辐照,分析辐照对种子萌发、幼苗生长和叶片生理指标的影响.结果表明:60Co-γ射线辐照抑制种子萌发及幼苗生长,且剂量越大,抑制作用越强;低剂量(≤300 Gy)60Co-γ射线辐照阻碍幼苗叶片的可溶性糖积累,高剂量(＞300 Gy)60Co-γ射线辐照促进幼苗叶片的可溶性糖积累;60Co-γ射线辐照可提高幼苗叶片的可溶性蛋白含量及叶绿素含量,其中,剂量为 200 Gy时增加最多.石榴种子的最适辐射剂量范围为 268.22～322.54 Gy.

Abstract

The seeds of Tunisia soft seed were irradiated with 100 Gy,200 Gy,300 Gy and 400 Gy by 60Co-γ irradiation,and the effects of irradiation on seed germination,seedling growth and leaf physiology and biochemistry were analyzed.The results showed that 60Co-γ irradiation inhibited seed germination and seedling growth,and the greater the dose,the stronger the inhibition effect was.Low dose(≤300 Gy)60Co-γ irradiation inhibited the accumulation of soluble sugar in seedling leaves,while high dose(>300 Gy)60Co-γ irradiation promoted the accumulation of soluble sugar in seedling leaves.The contents of soluble protein and chlorophyll in leaves of seedlings were increased by irradiation with 60Co-γ irradiation,and the contents of soluble protein and chlorophyll were increased most at 200 Gy.The optimal radiation dose range of pomegranate seeds was 268.22～322.54 Gy.

关键词

诱变育种/石榴/种子萌发/60Co-γ辐射

Key words

mutation breeding/pomegranate/seed germination/60Co-γ radiation

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基金项目

河南省科技攻关项目(232102110224)

出版年

2024

中国果树

中国农业科学院果树研究所

中国果树

CSTPCD

影响因子：0.45

ISSN：1000-8047

参考文献量34

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