Detection and pathogenicity analysis of Erwinia amylovorabacteria in overwintering plant debris of rosaceae plants
In order to determine the origin of the initial infection of pear fire blight in Xinjiang.The overwintering disease residues of 6 species of rosae were collected from Korla City and Luntai County of Bazhou,Huocheng County and Yining City of Yili Prefecture.Using the methods of plate isolation and streak culture,pathogenicity test,PCR detection and sequence analysis,the existence and activity of pear fire blight in the diseased remains were studied.The results showed that the pathogenic bacteria could be isolated from the diseased tissues of the 6 host species,and the isolated pathogens could be pathogenic when inoculated on the isolated leaves,and were identified as pear fire blight by molecular biology.Erwinia amylovorawas found to be pathogenic in the remnants of 6 host species(flower rot,leaf rot,branch rot,fruit rot,ulcerative plaque tissue).Therefore,it provides a theoretical basis for timely removal of overwintering disease residues in orchards and prevention and control of the occurrence and prevalence of pear fire blight.
万方数据
维普
