Selection of kiwifruit pollinators from Actinidia chinensis strains
This study employs three male strains of Actinidia chinensis as experimental materials to quantify the pollen quantity per anther and assess the pollen viability of different strains.Additionally,it examines the impact of varying storage conditions on the in vitro pollen viability of these strains,thereby providing insights of the selection and breeding of kiwifruit pollinators.The results demonstrate that the pollen germination rate of the three tested strains can reach over 56％,with the 10-3 strain exhibiting notably higher pollen viability than the other two strains.On the day of flowering,the pollen viability of the 10-3 strain reached 78.47％,with the number of pollen grains per anther being 35,500.00 which is a significantly higher quantity than that observed in the other two strains.The viability of the pollen was measured following storage,and it was found that the highest viability was observed on the day of flowering for the three male flowers of the kiwifruit that were tested.Storage at 4 ℃ for 7 days had no significant impact on pollen viability.Following a 30-day storage period at 4 ℃,the pollen viability of the 5-3 and 10-3 strains declined to 37.65％and 44.03％,respectively.In contrast,the pollen viability of the 6-1 strain decreased to 2.25％,which is insufficient to meet the pollination requirements.Pollen viability of the 5-3 and 10-3 strains stored at-20 ℃ for 30 days and 60 days,respectively,remained above the production threshold for pollination and fruit setting,with viability percentages of 46.99％,53.14％and 42.78％,45.34％.However,the pollen viability of the 6-1 strain decreased to 16.14％and 23.24％after 15 days of storage at 4 ℃ and 30 days of storage at-20 ℃,respectively.It can therefore be concluded that the 5-3 and 10-3 strains exhibit high germination rates and a substantial quantity of pollen per anther,in addition to displaying robust cold storage viability.These attributes render them suitable candidates for use as pollination varieties.
万方数据
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