Preliminary study of physiological response evaluation of alkaline stress in 30 kiwifruit germplasms
Thirty tissue-cultured kiwifruit germplasm vines were used to study the effects of alkaline stress on plant growth phenotype,biomass,leaf photosynthesis and root antioxidant capacity,and iron(Fe)uptake and transport in various plant parts under sand culture condition.Moreover,principal component analysis,membership function method and cluster analysis were employed to totally assess the alkaline tolerance of different kiwifruit germplasms.This will provide evidence for elucidating the mechanism of alkaline tolerance and for breeding the rootstocks in kiwifruit species.The results showed that thirty-four out of thirty-eight index tested were significantly affected by alkaline stress,and fourteen index were greater than 0.6 in alkaline sensitivity.Nine representative index were selected by correlation analysis and significant test,such as plant height,leaf area,root fresh weight,whole plant dry weight,whole plant dry matter content,total Fe concentration in roots,ratio of stem to root in total Fe,ratio of leaf to stem in total Fe,and POD activity in roots.Furthermore,the nine index were analyzed by principal component analysis,weight-based membership function method and cluster analysis,to obtain four high alkaline-tolerance germplasms(GZ1,DE2,DE1 and DE7)and two high alkaline-sensitive germplasms(MW3 and MW13).
kiwifruitgermplasmsalkaline stressevaluation of alkaline toleranceprincipal component analysismembership function methodcluster analysis