Detection of resistance level and mutation frequency of resistance genes to common acaricides against the two spotted spider mite,Tetranychus urticae
Two spotted spider mite,Tetranychus urticae is a major agriculture pest,causing serious economic losses every year.To shed light on the IPM of T.urticae in apple orchard,the overall goal of this study is used vial-leaf dipping(VLD)method to determine the resistance level to three commercially available acaricides(abamectin,B-azolemiteacrylic,and bifenazate)in T.urticae field populations and the mutation frequency of resistance gene for avermectin in these populations.The results showed that the toxicity assay showed that the ranking of acaricidal activities from high to low was abamectin＞B-azolemiteacrylic＞bifenazate,and the field populations had the highest fold of resistance to abamectin.As for the mutation frequency of Glu Cl,Yuncheng population was the highest(98.94％),followed by Liquan(97.78％)and Shilin(1.09％)in 2019,and 100.00％(Yuncheng),98.91％(Liquan),and 1.06％(Shilin)in 2022.T.urticae field populations have a high risk of developing resistance to abamectin.Therefore,the use of abamectin for controlling of T.urticae in apple orchard should be cautious,and the resistance monitoring program should he strongly encouraged.
万方数据
维普
