Expression of Jilin White Goose Interferon - α in Escherichia coli and Preparation of Rabbit Antisera against Goose Interferon -α
The interferon a gene of Jilin white goose was amplified from genome DNA, the segment coding mature peptide of the GolFN -α gene was cloned into prokaryotic expression vector pGEX - 6p - 1 to construct recombinant plasmid pGEX - IFN -α. pGEX - IFN -α was transformed into E. coli BL - 21 ( DE3 ) , which can express fused protein GST - IFN -α in E. coli BL - 21 ( DE3 ) by IPTG inducing. The expressed product was identified by SDS - PAGE and Western blot. The results showed that fusion protein GST - IFN -α was about 45 ku and it could react with interferon antibody. After purificating with Glutathione Sepharose -4B affinity column, and then refolded by dialysis against water, antisera against goose IFN -a was generated by immunizing rabbis with the purified protein. The expression and purification of recombinant goose IFN - ct fusion protein and preparation of the antisera against goose IFN -α will lay a foundation for the future application of goose IFN -α.
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万方数据
维普
