Screening of Hemophilus parasuis Serotype 4 Strain
In order to screen the challenge strains of Hemophilus parasuis(HPS)serotype 4,8 strains were identified by serotype identification and the bacterial growth curve was drawn.Five strains were tested by guinea pig challenge test,and the clinical symptoms and pathological changes of infected guinea pigs were recorded.The results showed that all the 8 strains were HPS serotype 4,and the optimal extended culture time of candidate strains was determined to be 8~10 hours,and CVCC4355 with the strongest virulence was selected.In this study,CVCC4355 strain was identified as a challenge strain for testing the efficacy of HPS serotype 4 vaccine products.
Haemophilus parasuis serotype 4screenvirulence test