Study on Strains for Testing Pasteurella multocida in Poultry
In order to study the strains of Pasteurella multocida for the efficacy evaluation of veterinary biological products,a batch of avian Pasteurella multocida CVCC44801 strains were prepared,and the morphology,biochemical characteristics,culture characteristics,serological characteristics,vacuum degree,purity,residual water and virulence of the strains were tested,and the stability of the freeze-dried bacteria was further evaluated.The virulence of freeze-dried bacteria was compared with that of freshly cultured bacteria.The results showed that the morphology,biochemical characteristics,culture characteristics,serological characteristics,vacuum degree,purity,residual water and virulence of the lyophilized strain were in accordance with the quality standard of the Regulations of the People's Republic of China on Veterinary Biological Products 2000 edition.The number of freeze-dried bacteria remained stable,and the virulence was not different from that of freshly cultured bacteria.This study provides a reference for the preparation and verification of strains for the efficacy evaluation of Pasteurella multocida bioproducts for veterinary use.