红霉素高产菌株选育及其发酵条件的优化
Breeding of High Erythromycin-producing Strains and Optimization of Fermentation Conditions
李春玲 1牛莎莎 1牛春 1石彦鹏 1张萍1
作者信息
- 1. 宁夏泰瑞制药股份有限公司,银川 750101
- 折叠
摘要
为选育出红霉素(Erythromycin)发酵生产的优良菌株并建立其高效发酵条件,以产红霉素的红色糖多孢菌(Saccharopolyspora erythraea)SE-2207 菌株作为原始菌株,利用EMS(甲基磺酸乙酯)、UV(紫外诱变)和ARTP(常压室温等离子体诱变)诱变的方法开展优良菌种选育,比较了三种方法的诱变效果,选育出1 株遗传稳定、且对红霉素碱耐受性强的菌株SEM-7,其红霉素发酵效价达到8368 mg/L,比原始菌株提高了25.5%.而且,SEM-7 菌株细胞酰基辅酶A合成酶活力显著高于原始菌株.通过对发酵培养基中添加正丙醇、豆油的浓度进行优化筛选,使SEM-7 菌株500 L中试发酵效价达到9155 mg/L.研究结果对提升红霉素工业化发酵生产水平具有重要意义.
Abstract
In order to select a good strain and optimize fermentation processes for erythromycin fermentation,Saccharopolyspora erythraea SE-2207 strain producing erythromycin was used as the original strain.SE-2207 strain was mutagenized by mutagenesis EMS(ethyl methanesulfonate),UV(ultraviolet)and ARTP(atmospheric pressure room temperature plasmawas),respectively.And the mutagenic effects of the three methods were compared.After mutagenesis,SEY-7 strain with good genetic stability and high erythromycin base tolerance was screened out.Its mycophenolic acid fermentation potency was up to 8368 mg/L,which was 25.5%higher than that of the original strain.Based on screening concentration of N-propanol and soybean oil in the medium,the fermentation processes were optimized and made the fermentation titer of 500 L up to 9155 mg/L.The results were of great significance to improve fermentation production level of erythromycin.
关键词
红霉素/高产菌株/诱变/发酵条件/优化Key words
erythromycin/high yield strain/mutagenesis/fermentation processes/optimization引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据